先前的研究已经证明这些分子在乳腺癌细胞中具有抗雌激素和抗增殖作用。值得注意的是，我们报道了纯合成甘油蛋白 I 和 II 通过多种途径发挥作用，包括 ERα、FOXM1、AhR 和 HIF 途径，抑制细胞增殖和迁移。在这项研究中，通过与酱油曲霉进行固体发酵获得的粗大豆提取物中富含的甘油蛋白，在体内对植入鸡蛋绒毛尿囊膜中的MCF-7乳腺癌细胞和切除卵巢的裸鼠进行了体内潜在抗肿瘤活性的研究。第一项试验显示，用天然提取物处理的 MCF-7 细胞的迁移显着减少。然而，天然提取物显着降低了口服喂养裸鼠移植肿瘤的雌激素依赖性生长。我们的结果表明，天然大豆提取物轻微但显着地减少了口服喂养的裸鼠移植肿瘤的雌激素依赖性生长。 Ki-67 和组蛋白 H3S10 磷酸化 (H3S10P) 的免疫组织化学证实了这些结果，表明在饲喂发酵提取物的小鼠的移植肿瘤中，这些增殖标记物的表达较低。此外，与对照动物相比，我们观察到血管生成标记物（例如 CD31 和 CD34）的表达较低。令人惊讶的是，移植的 MCF-7 细胞的 RNA 转录组分析显示没有差异基因表达。这些结果可能表明口服天然甘油蛋白在全身发挥生物效应，间接减少肿瘤血管生成，从而减少肿瘤体积。总体而言，我们的结果表明，从大豆中提取的成分甘油蛋白在预防和治疗乳腺癌方面具有潜在的治疗应用。© 2024 作者。 《食品科学杂志》由 Wiley periodicals LLC 代表食品技术专家协会出版。

Previous studies have demonstrated antiestrogenic and antiproliferative effects of these molecules in breast cancer cells. Notably, we have reported that pure synthetic glyceollins I and II act through various pathways, including ERα, FOXM1, AhR, and HIF pathways to inhibit cell proliferation and migration. In this study, the potential antitumor activity of glyceollins enriched in crude soybean extracts, obtained by solid fermentation with Aspergillus sojae, was investigated in vivo on MCF-7 breast cancer cells implanted in the chorioallantoic membrane of the chick egg and on ovariectomized nude mice. The first trial showed a substantial reduction in the migration of MCF-7 cells treated with the natural extracts. However, the natural extracts significantly reduced the estrogen-dependent growth of transplanted tumors in orally fed nude mice. Our results showed that natural soybean extracts slightly but significantly reduced estrogen-dependent growth of the transplanted tumors in orally fed nude mice. These results were confirmed by immunohistochemistry of Ki-67 and histone H3S10 phosphorylation (H3S10P), revealing lower expression of these proliferation markers in the transplanted tumors from mice fed with the fermented extracts. Additionally, compared to the control animals, we observed a lower expression of angiogenesis markers such as CD31 and CD34. Surprisingly, transcriptomic analysis of RNA from transplanted MCF-7 cells revealed no differential gene expression. These results may suggest that orally consumed natural glyceollins exert biological effects throughout the body, acting indirectly to reduce tumor angiogenesis and consequently tumor volume. Overall, our results indicate that glyceollins, elicited components of the soy origin, hold potential therapeutic applications for the prevention and treatment of breast cancer.© 2024 The Author(s). Journal of Food Science published by Wiley Periodicals LLC on behalf of Institute of Food Technologists.