研究动态
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酶法改性竹笋膳食纤维对2型糖尿病大鼠的降血糖作用。

The hypoglycemic effect of enzymatic modified dietary fiber from bamboo shoot on type 2 diabetes rats.

发表日期:2024 Aug 16
作者: Xiangjie Yao, Renkou Wan, Chunxiao Li, Gongjing Li, Bing Zhang, Zeyuan Deng, Hongyan Li
来源: DIABETES & METABOLISM

摘要:

竹笋是富含膳食纤维(DF)的健康食品。然而,其高度不溶的DF和纤维质地限制了其在工业加工食品中的应用。为了实现竹笋的工业化加工,本研究利用纤维素酶来改善竹笋DF的物理特性。酶解后,竹笋可溶性DF(SDF)含量显着增加了99.28%(从5.53%增加到11.02%)(p < 0.01)。同时探讨酶改性竹SDF(EMBSDF)对链脲佐菌素诱导的2型糖尿病大鼠的作用。结果表明,与糖尿病大鼠相比,高剂量 EMBSDF (312.8 mg/kg) 治疗的大鼠在葡萄糖耐量和胰岛素敏感性方面表现出显着改善 (p < 0.01)。同时观察到,与糖尿病大鼠相比,高剂量EMBSDF的胰高血糖素样肽-1、脂联素和白细胞介素-4水平分别增加了57.79%、159.13%和6.17%(p < 0.01)。肿瘤坏死因子-α、C 反应蛋白和瘦素水平分别降低 (p < 0.01) 62.89%、31.53% 和 7.84%。此外,在高剂量和中剂量 EMBSDF 治疗的糖尿病大鼠中发现了明显的肾脏和胰腺组织学改善。这些结果表明,改良的 DF 显着改善了糖尿病。© 2024 Institute of Food Technologies。
Bamboo shoot is a healthy food rich in dietary fiber (DF). However, its highly insoluble DF and fibrous texture limit its application in industrially processed foods. To achieve industrial processing of bamboo shoot, cellulase was used to improve the physical characteristics of bamboo shoot DF in this study. After enzymatic hydrolysis, the content of soluble DF (SDF) of bamboo shoot increased by 99.28% (from 5.53% to 11.02%) significantly (p < 0.01). At the same time, the effect of enzymatic-modified bamboo SDF (EMBSDF) on streptozotocin-induced type 2 diabetes rats was explored. Results demonstrated that the high dose of EMBSDF (312.8 mg/kg) treated rats showed significant improvements in terms of glucose tolerance and insulin sensitivity (p < 0.01) compared with the diabetes rats. Meantime, it was observed that the levels of glucagon-like peptide-1, adiponectin and interleukin-4 of high dose of EMBSDF compared with diabetes rats were increased (p < 0.01) by 57.79%, 159.13%, and 6.17%, respectively. The tumor necrosis factor-α, C-reactive protein, and leptin levels were decreased (p < 0.01) by 62.89%, 31.53%, and 7.84%, respectively. Furthermore, apparent kidney and pancreas histology improvements were found in high-dose and mid-dose EMBSDF-treated diabetes rats. These results indicated that the modified DF significantly improved diabetes.© 2024 Institute of Food Technologists.