生物传感器研究长期以来一直致力于实现尽可能低的检测限 (LOD)，推动灵敏度的显着进步并开辟分析的新可能性。然而，这种对低 LOD 的强烈关注可能并不总是能满足实际需求或适合这些设备的实际用途。虽然技术进步令人印象深刻，但有时它们可​​能会忽视重要因素，例如检测范围、易用性和市场准备情况，这些因素对于生物传感器在实际应用中有效发挥至关重要。本综述提倡采用平衡的方法来开发生物传感器，强调需要将技术进步与实用性结合起来。我们深入研究了各种应用，包括癌症生物标志物、病理相关生物标志物和非法药物的检测，说明了 LOD 在这些背景下的关键作用。通过考虑临床需求和更广泛的设计方面（例如成本效益、可持续性和法规遵从性），我们认为将技术进步与实用性相结合将增强生物传感器的影响。这种方法确保生物传感器不仅技术可靠，而且在实际应用中具有广泛的用途和益处。解决各种分析参数以及用户期望和市场需求可能会最大限度地发挥生物传感器的现实影响。版权所有 © 2024 Elsevier B.V. 保留所有权利。

Biosensor research has long focused on achieving the lowest possible Limits of Detection (LOD), driving significant advances in sensitivity and opening up new possibilities in analysis. However, this intense focus on low LODs may not always meet the practical needs or suit the actual uses of these devices. While technological improvements are impressive, they can sometimes overlook important factors such as detection range, ease of use, and market readiness, which are vital for biosensors to be effective in real-world applications. This review advocates for a balanced approach to biosensor development, emphasizing the need to align technological advancements with practical utility. We delve into various applications, including the detection of cancer biomarkers, pathology-related biomarkers, and illicit drugs, illustrating the critical role of LOD within these contexts. By considering clinical needs and broader design aspects like cost-effectiveness, sustainability, and regulatory compliance, we argue that integrating technical progress with practicality will enhance the impact of biosensors. Such an approach ensures that biosensors are not only technically sound but also widely useable and beneficial in real-world applications. Addressing the diverse analytical parameters alongside user expectations and market demands will likely maximize the real-world impact of biosensors.Copyright © 2024 Elsevier B.V. All rights reserved.