使用具有强等离子等离激元纳米界面的多路复用双光学微纤维，同时对前列腺特异性抗原和lncRNA PCA3的非侵入性超敏感检测
Simultaneous noninvasive ultrasensitive detection of prostate specific antigen and lncRNA PCA3 using multiplexed dual optical microfibers with strong plasmonic nanointerfaces
影响因子:10.50000
分区:化学1区 Top / 生物物理1区 生物工程与应用微生物1区 分析化学1区 电化学2区
发表日期:2024 Nov 15
作者:
Hongtao Li, Xu Wang, Hao Wu, Weisheng Wang, Aiyun Zheng, Jun Zhu, Lili Liang, Huojiao Sun, Liang Lu, Jialiang Lv, Qi Yu, Hongzhi Wang, Benli Yu
摘要
诊断前列腺癌（PCA）的准确性低，这很容易由仅测定单一前列腺特异性抗原（PSA）生物标志物引起。尽管传统报道的同时检测两个特定PCA生物标志物的方法可以提高诊断效率和准确性，但低检测敏感性限制了它们在极端阶段PCA临床测定应用中的使用。为了克服上述缺点，本文提出了用金纳米棒（GNR）（GNR）和Au纳米果胶（AU NBPS）纳米界面分别官能化具有强大局部局部表面等离子体共振（LSPR）效应的纳米肌膜。这些传感器可以同时检测PSA蛋白生物标志物和长的非编码RNA前列腺癌抗原3（LNCRNA PCA3）具有超高敏感性和显着的特异性。因此，所提出的双光学微纤维多路复用生物传感器可以检测PSA蛋白和LNCRNA PCA3，具有3.97×10-15 mol/l的超低限制（LODS）的超低限制限制（LODS），在纯磷酸盐溶液中（PBS）中，分别是较低的，分别是在纯phosphorus haster溶液中，分别是三分之一的摩rone/l。 PCA测定技术。此外，传感器可以将目标成分与复杂的生理环境区分开，从而显示出明显的传感器生物传感特异性。通过传感器的表现良好，他们可以同时成功地分别在未稀释的人血清和尿液中分析PSA和LNCRNA PCA3。因此，我们提出的多重传感器可以同时实时高敏感性检测复杂的人类样本，从而为早期PCA个体的高性诊断提供了一种新颖的宝贵方法。
Abstract
Low accuracy of diagnosing prostate cancer (PCa) was easily caused by only assaying single prostate specific antigen (PSA) biomarker. Although conventional reported methods for simultaneous detection of two specific PCa biomarkers could improve the diagnostic efficiency and accuracy, low detection sensitivity restrained their use in extreme early-stage PCa clinical assay applications. In order to overcome above drawbacks, this paper herein proposed a multiplexed dual optical microfibers separately functionalized with gold nanorods (GNRs) and Au nanobipyramids (Au NBPs) nanointerfaces with strong localized surface plasmon resonance (LSPR) effects. The sensors could simultaneously detect PSA protein biomarker and long noncoding RNA prostate cancer antigen 3 (lncRNA PCA3) with ultrahigh sensitivity and remarkable specificity. Consequently, the proposed dual optical microfibers multiplexed biosensors could detect the PSA protein and lncRNA PCA3 with ultra-low limit-of-detections (LODs) of 3.97 × 10-15 mol/L and 1.56 × 10-14 mol/L in pure phosphorus buffer solution (PBS), respectively, in which the obtained LODs were three orders of magnitude lower than existed state-of-the-art PCa assay technologies. Additionally, the sensors could discriminate target components from complicated physiological environment, that showing noticeable biosensing specificity of the sensors. With good performances of the sensors, they could successfully assay PSA and lncRNA PCA3 in undiluted human serum and urine simultaneously, respectively. Consequently, our proposed multiplexed sensors could real-time high-sensitivity simultaneously detect complicated human samples, that providing a novel valuable approach for the high-accurate diagnosis of early-stage PCa individuals.