多路复用双光学微纤维结合强等离子体纳米界面实现前列腺特异抗原和lncRNA PCA3的非侵入式超高灵敏检测

Simultaneous noninvasive ultrasensitive detection of prostate specific antigen and lncRNA PCA3 using multiplexed dual optical microfibers with strong plasmonic nanointerfaces

BIOSENSORS & BIOELECTRONICS

影响因子:10.5

分区:化学1区 Top / 生物物理1区生物工程与应用微生物1区分析化学1区电化学2区

发表日期:2024 Nov 15

作者: Hongtao Li, Xu Wang, Hao Wu, Weisheng Wang, Aiyun Zheng, Jun Zhu, Lili Liang, Huojiao Sun, Liang Lu, Jialiang Lv, Qi Yu, Hongzhi Wang, Benli Yu

DOI: 10.1016/j.bios.2024.116672

摘要

单纯检测前列腺特异性抗原（PSA）生物标志物的准确性较低，容易导致前列腺癌（PCa）诊断偏差。虽然现有的同步检测两种PCa特异性生物标志物的方法能提高诊断效率和准确性，但其检测灵敏度不足，限制了其在极早期临床检测中的应用。为克服这些缺陷，本文提出一种多路复用的双光学微纤维传感器，分别用金纳米棒（GNRs）和金纳米二尖体（Au NBPs）功能化，利用强局域表面等离子体共振（LSPR）效应。这些传感器能同时高灵敏度、高特异性地检测PSA蛋白和长链非编码RNA前列腺癌抗原3（lncRNA PCA3），其极低的检测限分别为3.97×10^-15 mol/L和1.56×10^-14 mol/L，比现有的PCa检测技术低三个数量级。此外，传感器还能在复杂的生理环境中鉴别目标组分，表现出优异的生物传感特异性。在未稀释的血清和尿液中，传感器也能成功同时检测PSA和lncRNA PCA3，显示出实时高灵敏度检测复杂生物样本的潜力，为早期PCa的高精确诊断提供了一种新颖有效的方法。

Abstract

Low accuracy of diagnosing prostate cancer (PCa) was easily caused by only assaying single prostate specific antigen (PSA) biomarker. Although conventional reported methods for simultaneous detection of two specific PCa biomarkers could improve the diagnostic efficiency and accuracy, low detection sensitivity restrained their use in extreme early-stage PCa clinical assay applications. In order to overcome above drawbacks, this paper herein proposed a multiplexed dual optical microfibers separately functionalized with gold nanorods (GNRs) and Au nanobipyramids (Au NBPs) nanointerfaces with strong localized surface plasmon resonance (LSPR) effects. The sensors could simultaneously detect PSA protein biomarker and long noncoding RNA prostate cancer antigen 3 (lncRNA PCA3) with ultrahigh sensitivity and remarkable specificity. Consequently, the proposed dual optical microfibers multiplexed biosensors could detect the PSA protein and lncRNA PCA3 with ultra-low limit-of-detections (LODs) of 3.97 × 10-15 mol/L and 1.56 × 10-14 mol/L in pure phosphorus buffer solution (PBS), respectively, in which the obtained LODs were three orders of magnitude lower than existed state-of-the-art PCa assay technologies. Additionally, the sensors could discriminate target components from complicated physiological environment, that showing noticeable biosensing specificity of the sensors. With good performances of the sensors, they could successfully assay PSA and lncRNA PCA3 in undiluted human serum and urine simultaneously, respectively. Consequently, our proposed multiplexed sensors could real-time high-sensitivity simultaneously detect complicated human samples, that providing a novel valuable approach for the high-accurate diagnosis of early-stage PCa individuals.