仅检测单一前列腺特异性抗原（PSA）生物标志物很容易导致诊断前列腺癌（PCa）的准确性低。尽管传统报道的同时检测两种特定 PCa 生物标志物的方法可以提高诊断效率和准确性，但较低的检测灵敏度限制了它们在极早期 PCa 临床检测应用中的使用。为了克服上述缺点，本文提出了一种分别用具有强局域表面等离子体共振（LSPR）效应的金纳米棒（GNR）和金纳米双锥（Au NBP）纳米界面进行功能化的复用双光学微纤维。该传感器可以同时检测PSA蛋白生物标志物和长链非编码RNA前列腺癌抗原3（lncRNA PCA3），具有超高灵敏度和显着的特异性。因此，所提出的双光学微纤维复合生物传感器可以检测纯磷中的 PSA 蛋白和 lncRNA PCA3，检测限 (LOD) 超低，分别为 3.97 × 10-15 mol/L 和 1.56 × 10-14 mol/L分别使用缓冲溶液 (PBS) 进行检测，其中获得的 LOD 比现有最先进的 PCa 检测技术低三个数量级。此外，传感器可以从复杂的生理环境中区分目标成分，显示出传感器显着的生物传感特异性。凭借传感器的良好性能，他们可以成功地分别同时测定未稀释的人血清和尿液中的PSA和lncRNA PCA3。因此，我们提出的多路复用传感器可以实时高灵敏度同时检测复杂的人体样本，为早期 PCa 个体的高精度诊断提供一种新颖有价值的方法。版权所有 © 2024 Elsevier B.V. 保留所有权利。

Low accuracy of diagnosing prostate cancer (PCa) was easily caused by only assaying single prostate specific antigen (PSA) biomarker. Although conventional reported methods for simultaneous detection of two specific PCa biomarkers could improve the diagnostic efficiency and accuracy, low detection sensitivity restrained their use in extreme early-stage PCa clinical assay applications. In order to overcome above drawbacks, this paper herein proposed a multiplexed dual optical microfibers separately functionalized with gold nanorods (GNRs) and Au nanobipyramids (Au NBPs) nanointerfaces with strong localized surface plasmon resonance (LSPR) effects. The sensors could simultaneously detect PSA protein biomarker and long noncoding RNA prostate cancer antigen 3 (lncRNA PCA3) with ultrahigh sensitivity and remarkable specificity. Consequently, the proposed dual optical microfibers multiplexed biosensors could detect the PSA protein and lncRNA PCA3 with ultra-low limit-of-detections (LODs) of 3.97 × 10-15 mol/L and 1.56 × 10-14 mol/L in pure phosphorus buffer solution (PBS), respectively, in which the obtained LODs were three orders of magnitude lower than existed state-of-the-art PCa assay technologies. Additionally, the sensors could discriminate target components from complicated physiological environment, that showing noticeable biosensing specificity of the sensors. With good performances of the sensors, they could successfully assay PSA and lncRNA PCA3 in undiluted human serum and urine simultaneously, respectively. Consequently, our proposed multiplexed sensors could real-time high-sensitivity simultaneously detect complicated human samples, that providing a novel valuable approach for the high-accurate diagnosis of early-stage PCa individuals.Copyright © 2024 Elsevier B.V. All rights reserved.