通过手术活检建立的癌症干细胞（CSC）与人类环境非常相似，可用于研究疾病机制、遗传适应性和治疗评估。在这里，我们提出了从室管膜肿瘤中衍生原发性患者来源的 CSC 系的方案。我们描述了从手术干预和活检到分离室管膜瘤以获得培养物的必要步骤。然后，我们详细介绍细胞系增殖的程序并定义这些原发癌细胞系的特征。有关该协议的使用和执行的完整详细信息，请参阅 Michealraj 等人1。版权所有 © 2024 作者。由爱思唯尔公司出版。保留所有权利。

Cancer stem cells (CSCs) established from surgical biopsies closely mimic the human context and can be used to investigate disease mechanisms, genetic fitness, and therapeutic evaluation. Here, we present a protocol for the derivation of primary patient-derived CSC lines from ependymal tumors. We describe the necessary steps, from surgical intervention and biopsy to the dissociation of ependymomas to derive cultures. We then detail procedures for cell line propagation and define the characteristics of these primary cancer cell lines. For complete details on the use and execution of this protocol, please refer to Michealraj et al.1.Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.