内源性逆转录病毒（ERV）是脊椎动物基因组中古代逆转录病毒感染的残余物，并由后代遗传。 ERVs可以通过基因突变或重组产生致病病毒。家猫的 ERV（ERV-DC）通过病毒重组产生猫白血病病毒 D 亚型（FeLV-D）。在此，我们将 B1 号染色体上的 ERV-DC8 位点定性为具有感染性复制能力的原病毒。 ERV-DC8感染了多种细胞系，包括人类细胞。 ERV-DC8 的透射电子显微镜鉴定出病毒释放为 Gammaretrovirus。 ERV-DC8被鉴定为FeLV-D病毒干扰群，其病毒受体为猫铜转运蛋白1。插入多态性分析显示，ERV-DC8 在家猫中高度整合。这项研究强调了 ERV 与其宿主之间的作用、致病性和进化关系。版权所有 © 2024 作者。由爱思唯尔公司出版。保留所有权利。

Endogenous retroviruses (ERVs) are remnants of ancient retroviral infections in vertebrate genomes and are inherited by offspring. ERVs can produce pathogenic viruses through gene mutations or recombination. ERVs in domestic cats (ERV-DCs) generate feline leukemia virus subgroup D (FeLV-D) through viral recombination. Herein, we characterized the locus ERV-DC8, on chromosome B1, as an infectious replication-competent provirus. ERV-DC8 infected several cell lines, including human cells. Transmission electron microscopy of ERV-DC8 identified the viral release as a Gammaretrovirus. ERV-DC8 was identified as the FeLV-D viral interference group, with feline copper transporter 1 as its viral receptor. Insertional polymorphism analysis showed high ERV-DC8 integration in domestic cats. This study highlights the role, pathogenicity, and evolutionary relationships between ERVs and their hosts.Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.