Ag绿色合成和封装的橄榄叶颗粒的抗氧化剂和抗癌电位在HCT-116细胞上
Antioxidant and anti-cancer potentials of Ag green-synthesized and encapsulated olive leaves particles on HCT-116 cells
影响因子:8.50000
分区:生物学2区 Top / 生化与分子生物学2区 应用化学2区 高分子科学2区
发表日期:2024 Oct
作者:
Hanem M M Mansour, Mohamed G Shehata, Amira M G Darwish, Elsayed Elsayed Hafez, Marwa A Samy, Neveen M Abdelmotilib, Eman M Abdo
摘要
水提取物（OLE），乳清蛋白包裹的提取物（OLE/WPN）和银纳米颗粒（OLE/AG-NPS）是由Manzenllie和Picual品种的橄榄叶制备的。这些制剂是表征的，并评估了其对VERO和HCT-116结直肠细胞的抗氧化剂和生物活性。通过肿瘤坏死因子-α（TNF-α）和细胞色素C氧化酶（COX1）基因表达研究制剂的作用机理。 OLE/AG-NP显示出比OLE/WPN（229.83-310.67 nm）较小的粒径（14.23-15.53 nm），并且由于它们的高效率为-24.86至-27.90 mV，其聚集较低。这些制剂均未影响Vero细胞（IC50 =192.19-421.01μg/mL）的生存能力，但它们对HCT-116细胞（IC50 =50.76-196.54μg/ml）的细胞毒性作用，尤其是OLE/WPN。此外，通过上调COX1表达并下调TNF-α表达，来自PICUAL品种（OLE，OLE/WPN和OLE/AG-NPS）的制剂显示对结肠癌对HCT-116细胞的调节作用。因此，OLE/WPN和OLE/AG-NP对于具有潜在健康益处的工业应用可能是有希望的。
Abstract
Water extracts (OLE), whey protein encapsulated extracts (OLE/WPNs), and silver nanoparticles (OLE/Ag-NPs) were prepared from olive leaves of Manzenllie and Picual varieties. These preparations were characterized, and their antioxidant and biological activities on Vero and HCT-116 colorectal cells were assessed. The mechanism of action of the preparations was studied through tumor necrosis factor-α (TNF-α) and cytochrome C oxidase (Cox1) gene expression. OLE/Ag-NPs showed smaller particle sizes (14.23-15.53 nm) than OLE/WPNs (229.83-310.67 nm) and demonstrated lower aggregation due to their high Ƹ-potential of -24.86 to -27.90 mV. None of the preparations affected the viability of Vero cells (IC50 = 192.19-421.01 μg/mL), but they showed cytotoxic effects on HCT-116 cells (IC50 = 50.76-196.54 μg/mL), particularly OLE/WPNs. Moreover, the preparations from the Picual variety (OLE, OLE/WPNs, and OLE/Ag-NPs) showed regulatory effects against colon cancer on treated HCT-116 cells by upregulating Cox1 expression and downregulating TNF-α expression. Consequently, OLE/WPNs and OLE/Ag-NPs could be promising for industrial applications with potential health benefits.