抗氧化和抗癌潜能：绿合成并封装在橄榄叶粒子中的银纳米粒子对HCT-116细胞的作用
Antioxidant and anti-cancer potentials of Ag green-synthesized and encapsulated olive leaves particles on HCT-116 cells
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影响因子:8.5
分区:生物学2区 Top / 生化与分子生物学2区 应用化学2区 高分子科学2区
发表日期:2024 Oct
作者:
Hanem M M Mansour, Mohamed G Shehata, Amira M G Darwish, Elsayed Elsayed Hafez, Marwa A Samy, Neveen M Abdelmotilib, Eman M Abdo
DOI:
10.1016/j.ijbiomac.2024.134776
摘要
从Manzenllie和Picual品种的橄榄叶中制备了水提取物（OLE）、乳清蛋白包封提取物（OLE/WPNs）和银纳米粒子（OLE/Ag-NPs）。对这些制剂进行了表征，并评估了其抗氧化和在Vero细胞及HCT-116结直肠癌细胞上的生物活性。通过肿瘤坏死因子-α（TNF-α）和细胞色素C氧化酶（Cox1）基因表达，研究了其作用机制。OLE/Ag-NPs表现出较小的粒径（14.23-15.53 nm）优于OLE/WPNs（229.83-310.67 nm），且因其高的Zeta电位（-24.86至-27.90 mV）表现出较低的聚集倾向。所有制剂均未影响Vero细胞的存活率（IC50=192.19-421.01 μg/mL），但对HCT-116细胞表现出细胞毒性（IC50=50.76-196.54 μg/mL），尤其是OLE/WPNs。此外，来自Picual品种的制剂（OLE、OLE/WPNs和OLE/Ag-NPs）通过上调Cox1表达和下调TNF-α表达，对受试HCT-116细胞的结肠癌表现出调节作用。因此，OLE/WPNs和OLE/Ag-NPs可能在工业应用中具有潜在的健康益处。
Abstract
Water extracts (OLE), whey protein encapsulated extracts (OLE/WPNs), and silver nanoparticles (OLE/Ag-NPs) were prepared from olive leaves of Manzenllie and Picual varieties. These preparations were characterized, and their antioxidant and biological activities on Vero and HCT-116 colorectal cells were assessed. The mechanism of action of the preparations was studied through tumor necrosis factor-α (TNF-α) and cytochrome C oxidase (Cox1) gene expression. OLE/Ag-NPs showed smaller particle sizes (14.23-15.53 nm) than OLE/WPNs (229.83-310.67 nm) and demonstrated lower aggregation due to their high Ƹ-potential of -24.86 to -27.90 mV. None of the preparations affected the viability of Vero cells (IC50 = 192.19-421.01 μg/mL), but they showed cytotoxic effects on HCT-116 cells (IC50 = 50.76-196.54 μg/mL), particularly OLE/WPNs. Moreover, the preparations from the Picual variety (OLE, OLE/WPNs, and OLE/Ag-NPs) showed regulatory effects against colon cancer on treated HCT-116 cells by upregulating Cox1 expression and downregulating TNF-α expression. Consequently, OLE/WPNs and OLE/Ag-NPs could be promising for industrial applications with potential health benefits.