从 Manzenllie 和 Picual 品种的橄榄叶中制备水提取物 (OLE)、乳清蛋白封装提取物 (OLE/WPNs) 和银纳米颗粒 (OLE/Ag-NPs)。对这些制剂进行了表征，并评估了它们对 Vero 和 HCT-116 结直肠细胞的抗氧化和生物活性。通过肿瘤坏死因子-α（TNF-α）和细胞色素C氧化酶（Cox1）基因表达研究制剂的作用机制。 OLE/Ag-NPs 显示出比 OLE/WPNs (229.83-310.67nm) 更小的粒径 (14.23-15.53nm)，并且由于其 -24.86 至 -27.90mV 的高电位而表现出较低的聚集。这些制剂均不影响Vero细胞的活力(IC50=192.19-421.01μg/mL)，但它们对HCT-116细胞(IC50=50.76-196.54μg/mL)，特别是OLE/WPNs表现出细胞毒性作用。此外，Picual 品种（OLE、OLE/WPNs 和 OLE/Ag-NPs）的制剂通过上调 Cox1 表达和下调 TNF-α 表达，对治疗的 HCT-116 细胞显示出抗结肠癌的调节作用。因此，OLE/WPNs 和 OLE/Ag-NPs 有望用于具有潜在健康益处的工业应用。版权所有 © 2024。由 Elsevier B.V. 出版。

Water extracts (OLE), whey protein encapsulated extracts (OLE/WPNs), and silver nanoparticles (OLE/Ag-NPs) were prepared from olive leaves of Manzenllie and Picual varieties. These preparations were characterized, and their antioxidant and biological activities on Vero and HCT-116 colorectal cells were assessed. The mechanism of action of the preparations was studied through tumor necrosis factor-α (TNF-α) and cytochrome C oxidase (Cox1) gene expression. OLE/Ag-NPs showed smaller particle sizes (14.23-15.53 nm) than OLE/WPNs (229.83-310.67 nm) and demonstrated lower aggregation due to their high Ƹ-potential of -24.86 to -27.90 mV. None of the preparations affected the viability of Vero cells (IC50 = 192.19-421.01 μg/mL), but they showed cytotoxic effects on HCT-116 cells (IC50 = 50.76-196.54 μg/mL), particularly OLE/WPNs. Moreover, the preparations from the Picual variety (OLE, OLE/WPNs, and OLE/Ag-NPs) showed regulatory effects against colon cancer on treated HCT-116 cells by upregulating Cox1 expression and downregulating TNF-α expression. Consequently, OLE/WPNs and OLE/Ag-NPs could be promising for industrial applications with potential health benefits.Copyright © 2024. Published by Elsevier B.V.