蠕虫寄生虫是一组来自不同分类科的复杂后生动物。活体寄生虫从表面分泌的排泄分泌（ES）副产物似乎可以调节宿主对蠕虫感染的免疫反应。本研究旨在探讨来自蠕虫寄生虫的 ES 抗原对结直肠细胞活力的影响。用无菌 PBS 冲洗后，将线虫在磷酸盐缓冲盐水 (PBS x1) 中于 37°C 培养 24 小时。使用研钵和研杵，使用 PBS 将蠕虫剧烈压碎。提取获得的排泄性分泌（ES）抗原，使用0.22 µM过滤器过滤，并保存在-20°C下以供进一步测定。对于 LCMS，使用 Agilent ZORBAX Eclipse Plus C18 快速分离 HT 分析 100 µl 提取物。使用 CRC 细胞系 HCT 116 提取 ES 抗原（10 µg/ml 和 20 µg/ml）用于细胞活力研究。细胞活力和 MTT 测定按照 MTT 试剂盒中提到的方案进行。液相色谱和质谱(LCMS)数据表明ES抗原含有代谢化合物，即脂肪酸、氨基醇、吲哚、甾醇、糖苷和鞘氨醇。对于蛔虫 LCMS 分析，检测到大约 405 个代谢峰。其中，通过数据库检测到了 58 种化合物，同时检测到的几种化合物具有抗癌特性。 MTT测定表明，暴露24小时和48小时后，与对照组相比，所有处理的细胞均表现出细胞活力下降。初步研究表明，来自蛔虫的ES抗原具有一定的降低HCT116 CRC细胞系细胞活力的能力。需要进一步的研究来检查 ES 抗原对 CRC 细胞系的细胞周期停滞和凋亡作用。

Helminth parasites are a group of complex metazoans from various taxonomic families. Excretory secretory (ES) by-products, secreted by living parasites from the surface, appeared to modulate the host immunological response towards helminth infection. This study aims to investigate the effect of ES antigen from helminth parasite on colorectal cell viability. Worm were cultured in phosphate-buffered saline (PBS x1) at 37°C for 24 hours after being rinsed in sterile PBS. Using a mortar and pestle, the worm was crushed vigorously using PBS. The obtained excretory secretory (ES) antigens were extracted and filtered using a 0.22 µM filter and stored at -20°C for further assay. For LCMS, 100 µl of the extract was analysed using Agilent ZORBAX Eclipse Plus C18 Rapid Resolution HT. The extraction of ES antigen (10 µg/ml and 20 µg/ml) was used for cell viability studies using CRC cell line HCT 116. Cell viability and MTT assay were conducted as per the protocol mentioned in the MTT kit. The liquid chromatography and mass spectrometry (LCMS) data indicated that the ES antigen contained metabolic compounds, namely fatty acid, amino alcohol, indoles, sterols, glycosides, and sphingoids. For the Ascaris lumbricoides LCMS analyses, around 405 metabolic peaks were detected. Out of which, 58 were detected via the database were identified, while several compounds detected have anticancer properties. The MTT assay indicated that after 24 hours and 48 hours of exposure, all treated cells showed a decrease in cell viability compared to the control group. The preliminary studies demonstrated that the ES antigen from Ascaris lumbricoides has some ability to decrease the cell viability of the HCT116 CRC cell line. Further studies are needed to examine the cell cycle arrest and apoptosis effect of the ES antigen towards the CRC cell line.