光生物调节利用可见光和近红外 (NIR) 光谱中的非电离光，被认为是增强组织修复、减少炎症并可能减轻癌症治疗相关副作用的潜在方法。近红外光被认为在细胞内被吸收，主要被线粒体内的发色团吸收。本研究探讨了 734 nm 近红外光对细胞衰老的影响。癌症（MCF7 和 A549）和非癌症（MCF10A 和 IMR-90）细胞群接受 63 mJ/cm2 近红外光照射 6 天。通过测量活性衰老相关β-半乳糖苷酶来量化衰老水平。暴露于近红外光会显着增加癌症细胞的衰老水平 (10.0%-203.2%)，但不会增加非癌细胞的衰老水平 (p > 0.05)。衰老的变化与线粒体稳态的显着调节有关，包括近红外光处理后活性氧水平（p<0.05）和线粒体膜电位（p<0.05）的增加。这些结果表明近红外光调节细胞化学，通过衰老诱导阻止癌细胞增殖，同时保护非癌细胞。© 2024 作者。 《生物光子学杂志》由 Wiley‐VCH GmbH 出版。

Photobiomodulation, utilising non-ionising light in the visible and near-infrared (NIR) spectrum, has been suggested as a potential method for enhancing tissue repair, reducing inflammation and possibly mitigating cancer-therapy-associated side effects. NIR light is suggested to be absorbed intracellularly, mainly by chromophores within the mitochondria. This study examines the impact of 734 nm NIR light on cellular senescence. Cancer (MCF7 and A549) and non-cancer (MCF10A and IMR-90) cell populations were subjected to 63 mJ/cm2 NIR-light exposure for 6 days. Senescence levels were quantified by measuring active senescence-associated beta-galactosidase. Exposure to NIR light significantly increases senescence levels in cancer (10.0%-203.2%) but not in non-cancer cells (p > 0.05). Changes in senescence were associated with significant modulation of mitochondrial homeostasis, including increased levels of reactive oxygen species (p < 0.05) and mitochondrial membrane potential (p < 0.05) post-NIR-light treatment. These results suggest that NIR light modulates cellular chemistry, arresting the proliferation of cancer cells via senescence induction while sparing non-cancer cells.© 2024 The Author(s). Journal of Biophotonics published by Wiley‐VCH GmbH.