本研究旨在探讨癌睾丸抗原家族成员 Semenogelin 1 (SEMG1) 在口腔鳞状细胞癌 (OSCC) 中的表达和生物学意义。此外，我们探讨了其与代谢相关分子的潜在关联。通过免疫组织化学、流式细胞术和蛋白质印迹分析测定了 OSCC 中 SEMG1 的表达水平。为了破译 SEMG1 在 OSCC 中的生物学意义，CAL27 OSCC 细胞系要么稳定过表达 SEMG1，要么进行 SEMG1-shRNA 敲低。还评估了 OSCC 患者临床病理参数与 SEMG1 表达之间的关系。发现 SEMG1 在 OSCC 中过表达，但其表达不受病理分级的影响。荧光二氢乙锭测定表明 SEMG1 增强了活性氧的产生。线粒体膜电位测定表明 SEMG1 显着上调线粒体膜电位。细胞周期评估强调，SEMG1 过度表达导致进入 S 期的细胞显着增加。此外，在 OSCC 中观察到 SEMG1 表达与 ENO1 和 PKM2 表达之间存在很强的相关性。研究结果强调了 SEMG1 在 OSCC 中的表达升高及其在 OSCC 患者肿瘤发生中的贡献作用。© 2024 John Wiley

This study aimed to investigate the expression and biological significance of Semenogelin 1 (SEMG1), a member of the cancer-testis antigen family, in oral squamous cell carcinoma (OSCC). Further, we explored its potential association with metabolism-related molecules.SEMG1 expression levels in OSCC were determined through immunohistochemistry, flow cytometry, and Western blot analyses. To decipher the biological implications of SEMG1 in OSCC, the CAL27 OSCC cell line was either stably overexpressed with SEMG1 or subjected to SEMG1-shRNA knockdown. The relationship between clinicopathological parameters and SEMG1 expression in OSCC patients was also assessed.SEMG1 was found to be overexpressed in OSCC, though its expression was not influenced by the pathological grade. The fluorescent dihydroethidium assay indicated that SEMG1 augmented reactive oxygen species production. The mitochondrial membrane potential assay suggested a significant upregulation of mitochondrial membrane potential by SEMG1. Cell cycle assessments highlighted that SEMG1 overexpression led to a notable rise in cells entering the S-phase. Additionally, a strong correlation between SEMG1 expression and both ENO1 and PKM2 expression in OSCC was observed.The findings underscore the elevated expression of SEMG1 in OSCC and its contributory role in the tumorigenesis of OSCC patients.© 2024 John Wiley & Sons Ltd.