放射治疗是肺癌有效的局部治疗方法。然而，基因与放射治疗之间的相互作用是多方面且错综复杂的。因此，我们探讨了miR-93-5p在A549细胞增殖、凋亡和迁移能力中的作用。同时，我们还研究了miR-93-5p与电离辐射（IR）之间的相互作用。通过Cell Counting Kit-8、Transwell和凋亡实验来测量增殖、迁移和凋亡能力。使用starBase v3.0预测肺癌中miR-93-5p及其靶基因的表达水平。然后，使用qPCR和western blot验证数据。miR-93-5p显着促进A549细胞的增殖（P < 0.01）和迁移能力（P < 0.001）。 Gasdermin E (GSDME) 被确定为 miR-93-5p 的假定靶标，并且与 miR-93-5p 呈负相关（P < 0.001）。 miR-93-5p 的过表达显着降低了 A549 中的 GSDME（P < 0.001）。有趣的是，在暴露于 IR 后，miR-93-5p 降低了 A549 细胞的细胞增殖 (P < 0.01) 和细胞迁移 (P < 0.01)，并增加了细胞凋亡 (P < 0.01)。miR-93-5p 被认为具有致癌作用。通过增强 A549 细胞增殖和迁移在肺癌中的作用。它可以增强IR条件下放疗的敏感性。我们推测，当细胞暴露于红外线时，miR-93-5p/GSDME 通路受到抑制，从而激活 GSDME 相关的细胞焦亡通路。因此，miR-93-5p可以克服放疗耐药，提高放疗疗效。Copyright © 2024 Qingtao Ni et al.

Radiation therapy is an effective local therapy for lung cancer. However, the interaction between genes and radiotherapy is multifaceted and intricate. Therefore, we explored the role of miR-93-5p in the proliferation, apoptosis, and migration abilities of A549 cells. Simultaneously, we also investigated the interactions between miR-93-5p and ionizing radiation (IR).Cell Counting Kit-8, transwell, and apoptotic assay were performed to measure the proliferation, migration, and apoptosis abilities. The expression levels of miR-93-5p and its target gene in lung cancer were predicted using starBase v3.0. Then, data were validated using qPCR and western blot.miR-93-5p significantly promoted the proliferation (P < 0.01) and migration abilities (P < 0.001) of A549 cells. Gasdermin E (GSDME) was identified to be a putative target of miR-93-5p and had a negative correlation with miR-93-5p (P < 0.001). Overexpression of miR-93-5p significantly decreased GSDME in A549 (P < 0.001). Interestingly, miR-93-5p decreased cell proliferation (P < 0.01) and cell migration (P < 0.01) and increased apoptosis (P < 0.01) in A549 cells after exposure to IR.miR-93-5p is presumed to play an oncogenic role in lung cancer by enhancing A549 cell proliferation and migration. It can enhance the sensitivity of radiotherapy under IR conditions. We speculate that the miR-93-5p/GSDME pathway was inhibited, activating the GSDME-related pyroptosis pathway when the cells were exposed to IR. Therefore, miR-93-5p can overcome resistance to radiotherapy and improve the efficacy of radiotherapy.Copyright © 2024 Qingtao Ni et al.