基于电化学适体（E-AB）的传感器可实现生物医学相关目标的检测和定量，例如生物样品中的小分子药物和蛋白质生物标志物。 E-AB 通常在市售的大电极上制造，虽然可用于快速传感器原型制作，但成本高昂，并且与生物样本库中通常用于临床验证研究的微升样品体积不兼容。为了开发一个多点传感平台，用于临床研究样本量特征的传感器验证，我们报告了 3D 打印 E-AB 的内部组装协议。我们采用商用 3D 立体光刻打印机（FormLabs，5000 美元）来制造电化学电池，并将电极直接嵌入 3D 打印的电池结构中。这种方法提供了可重复且可重复使用的电极制造过程，从而产生四个独立且同时的测量结果，以获得统计加权结果。我们证明了与结合抗生素和抗肿瘤药物的适体序列的兼容性。我们还展示了使用临床样本对血清万古霉素测量进行的概念验证。我们的结果表明，3D 打印可以与 E-AB 结合使用，对生物基质中的 E-AB 传感器进行可访问、快速且具有统计意义的验证。该期刊版权所有 © 英国皇家化学学会。

Electrochemical aptamer-based (E-AB) sensors achieve detection and quantitation of biomedically relevant targets such as small molecule drugs and protein biomarkers in biological samples. E-ABs are usually fabricated on commercially available macroelectrodes which, although functional for rapid sensor prototyping, can be costly and are not compatible with the microliter sample volumes typically available in biorepositories for clinical validation studies. Seeking to develop a multi-point sensing platform for sensor validation in sample volumes characteristic of clinical studies, we report a protocol for in-house assembly of 3D-printed E-ABs. We employed a commercially available 3D stereolithographic printer (FormLabs, $5k USD) for electrochemical cell fabrication and directly embedded electrodes within the 3D-printed cell structure. This approach offers a reproducible and reusable electrode fabrication process resulting in four independent and simultaneous measurements for statistically weighted results. We demonstrate compatibility with aptamer sequences binding antibiotics and antineoplastic agents. We also demonstrate a proof-of-concept validation of serum vancomycin measurements using clinical samples. Our results demonstrate that 3D-printing can be used in conjunction with E-ABs for accessible, rapid, and statistically meaningful validation of E-AB sensors in biological matrices.This journal is © The Royal Society of Chemistry.