锇(II)复合物因其抗癌活性、有利于癌细胞内生物转化的氧化还原电位及其在近红外（NIR）区域的发光特性，对潜在的治疗诊断剂具​​有有吸引力的特性。为了实现局部检测和递送，金纳米颗粒 (AuNP) 提供了一个有吸引力的支架，可以将多种发光剂附着在单个颗粒上，并为检测和局部递送提供多模式平台。我们开发了13 nm和25 nm AuNP，装饰有基于1,10-菲啉和表面活性联吡啶配体的锇络合物，OsPhenSS用于活细胞成像和单线态氧生成，标记为OsPhenSS·AuNP13和OsPhenSS·AuNP25。 AuNP 设计不仅允许探针定位的多种方式，而且还允许锇金属络合物的水溶性。锇修饰的纳米颗粒 OsPhenSS·AuNP13 和 OsPhenSS·AuNP25 在可见光激发的水溶液中显示出锇(II) 3MLCT 在 785 nm 处的特征性近红外发光。在肺癌和乳腺癌中孵育纳米颗粒后，锇的发光特征和金反射揭示了细胞质和核周区室中的定位。在 ROS 指示剂存在的情况下，在 552 nm 处激发纳米颗粒显示指示剂的绿色荧光显着增加，这与光诱导 ROS 的生成一致。通过锇和纳米颗粒探针的时间分辨发光研究检测单线态氧进一步证明了锇基纳米探针在成像和治疗方面的双重活性。引入用于携带锇成像探针的金纳米粒子，使得一种新颖的多功能策略将纳米级的检测和局部治疗相结合。

Osmium(II) complexes have attractive properties for potential theranostic agents given their anticancer activitiy, their redox potentials favourable for biological transformations within cancer cells and their luminescence in the near infrared (NIR) region. To achieve localised detection and delivery, gold nanoparticles (AuNP) provide an attractive scaffold to attach multiple luminescent agents on a single particle and provide a multimodal platform for detection and loaclaised delivery. We have developed 13 nm and 25 nm AuNP decorated with an osmium complex based on 1,10-phenantholine and surface active bipyridine ligands, OsPhenSS for live cell imaging and singlet oxygen generation, notated as OsPhenSS·AuNP13 and OsPhenSS·AuNP25. The AuNP designs not only allow versatile modalities for localisation of the probe but also water solubility for the osmium metal complex. The osmium decorated nanoparticles OsPhenSS·AuNP13 and OsPhenSS·AuNP25 display characteristic NIR luminescence from the osmium(II) 3MLCT at 785 nm in aqueous solutions with visible excitation. Upon incubation of the nanoparticles in lung cancer and breast carcinoma the luminescence signature of osmium and the gold reflectance reveal localisation in the cytoplasmic and perinuclear compartments. Excitation of the nanoparticles at 552 nm in the presence of a ROS indicator revealed a marked increase in the green fluorescence from the indicator, consistent with photo-induced ROS generation. The detection of singlet oxygen by time-resolved luminescence studies of the osmium and the nanoparticle probes further demonstrates the dual activity of the osmium-based nanoprobes for imaging and therapy. The introduction of gold nanoparticles for carrying osmium imaging probes allows a novel versatile strategy combining detection and localised therapies at the nanoscale.