岩藻黄质 (FX) 是一种非维生素原 A 类胡萝卜素，是食用褐藻中含有的众所周知的主要叶黄素。 FX 的纳米封装因其多种活性而受到推动。本研究采用乳清蛋白和亚麻籽胶作为生物大分子载体材料，按照我们早期的方法制备了纳米胶囊FX（nano-FX），并研究了nano-FX对异种移植小鼠的体内抗肿瘤作用和机制。 30只4周龄雄性BALB/c裸鼠适应性喂养7天，建立Huh-7细胞异种移植瘤模型。荷瘤小鼠服用nano-FX（50、25和12.5mgkg-1）和盐酸阿霉素（DOX，1mgkg-1）或不服用（对照）21天，n=6。 nano-FX的抑制率高达54.67±1.04%。 Nano-FX干预通过下调p-JNK、p-ERK、PI3Kp85α、p-AKT、p-p38MAPK、Bcl-2、CyclinD1和Ki-67的表达促进肿瘤组织细胞凋亡并诱导深染固缩和局灶性坏死，同时上调 cleaved caspase-3 和 Bax 的表达。 Nano-FX以剂量依赖性方式抑制肿瘤生长，保护荷瘤小鼠肝功能，上调凋亡相关蛋白水平，抑制MAPK-PI3K/Akt通路，促进肿瘤细胞凋亡。版权所有©2024爱思唯尔 B.V. 出版

Fucoxanthin (FX), a non-provitamin-A carotenoid, is a well-known major xanthophyll contained in edible brown algae. The nanoencapsulation of FX was motivated due to its multiple activities. Here, nano-encapsulated-FX (nano-FX) was prepared according to our early method by using whey protein and flaxseed gum as the biomacromolecule carrier material, then in vivo antitumor effect and mechanism of nano-FX on xenograft mice were investigated. Thirty 4-week-old male BALB/c nude mice were fed adaptively for 7 days to establish xenograft tumor model with Huh-7 cells. The tumor-bearing mice consumed nano-FX (50, 25, and 12.5 mg kg-1) and doxorubicin hydrochloride (DOX, 1 mg kg-1) or did not consume (Control) for 21 days, n = 6. The tumor inhibition rates of nano-FX were as high as 54.67 ± 1.04 %. Nano-FX intervention promoted apoptosis and induced hyperchromatic pyknosis and focal necrosis in tumor tissue by down-regulating the expression of p-JNK, p-ERK, PI3Kp85α, p-AKT, p-p38MAPK, Bcl-2, CyclinD1 and Ki-67, while up-regulating the expression of cleaved caspase-3 and Bax. Nano-FX inhibited tumor growth and protected liver function of tumor bearing mice in a dose-dependent manner, up-regulate the level of apoptosis-related proteins, inhibit the MAPK-PI3K/Akt pathways, and promote tumor cell apoptosis.Copyright © 2024. Published by Elsevier B.V.