目的探讨异荭草素对人胃癌细胞（HGC27细胞）凋亡、增殖、侵袭和迁移的影响。我们利用网络药理学来预测药物和疾病的靶点。采用CCK-8测定法测定异荭草素对HGC27细胞增殖的影响。采用流式细胞术分析异荭草素对HGC27细胞凋亡及细胞周期分布的影响。进行划痕试验和Transwell小室试验分别评估异荭草素对侵袭和迁移的影响。此外，通过qPCR和western blot分别检测异荭草素对凋亡相关基因和蛋白表达的影响。与对照组相比，异荭草素显着抑制HGC27细胞的增殖、迁移和侵袭。此外，Isoorientin 通过上调 Bax 和 caspase-3 的相对表达，同时下调 p-PI3K、p-AKT 和 Bcl-2 蛋白的相对表达来诱导 HGC27 细胞凋亡。 HGC27 细胞的迁移和迁移，并诱导胃癌细胞凋亡。© 2024。作者。

To investigate the effects of Isoorientin on the apoptosis, proliferation, invasion, and migration of human gastric cancer cells (HGC27 cells).We used network pharmacology to predict the targets of drugs and diseases. The CCK-8 assay was used to determine the effects of Isoorientin on the proliferation of HGC27 cells. Flow cytometry was employed to analyze the effects of Isoorientin on cell apoptosis and cell cycle distribution of HGC27 cells. Scratch test and transwell chamber test were conducted to assess the effects of Isoorientin on invasion and migration, respectively. Additionally, qPCR and western blot were performed to examine the impact of Isoorientin on apoptosis-related genes and protein expression, respectively.The Isoorientin significantly inhibited the proliferation, migration, and invasion of HGC27 cells compared to the control group. Furthermore, Isoorientin induced apoptosis in HGC27 cells by upregulating the relative expression of Bax and caspase-3 while downregulating the relative expression of p-PI3K, p-AKT, and Bcl-2 proteins.The Isoorientin exhibits inhibitory effects on the proliferation, invasion, and migration of HGC27 cells, and induces apoptosis in gastric cancer cells.© 2024. The Author(s).