膀胱癌是一种高发的恶性肿瘤。积雪草苷（AC）是一种三萜类化合物衍生物，对多种肿瘤具有抗肿瘤作用。本研究旨在探讨AC对膀胱癌的作用及机制。 J82和T24细胞用AC和/或丙泊酚处理，裸鼠皮下注射T24细胞。通过细胞计数试剂盒8、Transwell、流式细胞术、酶联免疫吸附试验、免疫组织化学和体内外实验探讨AC和/或丙泊酚的作用和机制。 AC抑制J82和T24细胞的细胞活力，其IC50值分别为2.43μM和2.16μM，丙泊酚抑制其IC50值分别为42.51μM和48.37μM。在动物和细胞实验中，单独使用 AC 或丙泊酚可降低细胞增殖、侵袭和免疫逃逸，并增加铁死亡，并下调 PI3K/AKT 通路的水平。异丙酚对上述指标的作用在体内外与AC共同作用下进一步增强。总而言之，AC 促进了丙泊酚对 PI3K/AKT 通路涉及的膀胱癌的改善作用。© 2024 Wiley periodicals LLC。

Bladder cancer is a highly prevalent malignancy. Asiaticoside (AC), a triterpenoid derivative, exhibits antitumor effect on different tumors. This study aimed to explore the role and mechanism of AC on bladder cancer. J82 and T24 cells were treated with AC and/or propofol, and nude mice were subcutaneously administrated with T24 cells. The effect and mechanism of AC and/or propofol were explored by cell counting kit-8, transwell, flow cytometry, enzyme-linked immunosorbent assay, immunohistochemistry and western blot assays both in vitro and in vivo. Cell viability of J82 and T24 cells was inhibited by AC with a IC50 value of 2.43 μM and 2.16 μM, and by propofol with a IC50 value of 42.51 μM and 48.37 μM, respectively. AC or propofol alone decreased cell proliferation, invasion, and immune escape with the increased ferroptosis, as well as downregulating the level of the PI3K/AKT pathway in both animal and cell experiments. The effect of propofol on the above-mentioned indicators was further enhanced with the co-treatment of AC in vitro and in vivo. Taken together, AC promoted the ameliorative effect of propofol on bladder cancer involved in PI3K/AKT pathway.© 2024 Wiley Periodicals LLC.