本研究评估了将诱导多能干细胞 (iPSC) 衍生的视网膜类器官移植到晚期色素性视网膜炎患者体内，使用自适应光学相干断层扫描 (AO-OCT) 监测移植后两年内的视网膜变化。我们的结果证实了成功的植入和增加的视网膜厚度，AO-OCT 提供了细胞结构的详细可视化，例如外部丛状层状线和玫瑰花状结构内的高反射颗粒，表明光感受器的发育。平行猴模型中的免疫组织学分析证实这些结构是成熟的、有功能的光感受器玫瑰花结。高分辨率 AO-OCT 与免疫组织学的整合为移植的结构和功能结果提供了重要的见解，代表了视网膜退行性疾病治疗方面的一个有希望的进步。版权所有 © 2024，Ishikura 等人。

This study evaluates the transplantation of induced pluripotent stem cell (iPSC)-derived retinal organoids into patients with advanced retinitis pigmentosa using adaptive optics optical coherence tomography (AO-OCT) to monitor retinal changes over two years post transplantation. Our results confirmed successful engraftment and increased retinal thickness, with AO-OCT providing detailed visualization of cellular structures such as an outer plexiform layer-like line and highly reflective particles within rosette-like formations, indicative of photoreceptor development. Immunohistological analysis in a parallel monkey model confirmed these structures as mature, functional photoreceptor rosettes. The integration of high-resolution AO-OCT with immunohistology provides critical insights into the structural and functional outcomes of transplantation and represents a promising advancement in the treatment of retinal degenerative diseases.Copyright © 2024, Ishikura et al.