肺癌是威胁人类健康最严重的恶性肿瘤。传统的临床前模型存在一定的缺陷；例如，它们不能准确反映肺癌的特征，而且其开发成本高且耗时。癌症干细胞（CSC）通过自组织产生与肺癌组织结构相似的癌类器官，在一定程度上克服了上述挑战，从而使其具有更广阔的应用前景。肺癌类器官（LCO）开发方法根据细胞来源可分为三大类，包括细胞系、患者来源的异种移植物和患者肿瘤组织/胸腔积液。已经描述了 17 种不同的 LCO 开发方法。根据细胞来源、使用的预处理方法、培养基和培养支架的组成，这些方法可以进一步合并为六类。这些类别是： i) 由特定转录因子诱导的 CSC； ii) 悬浮培养； iii) 相对最佳培养基； iv) 次优培养基； v) 机械消化和次优培养基； vi) 水凝胶支架。在当前的综述中，总结了上述每种方法的优点和缺点，并引用了支持研究的参考文献。版权所有：© 2024 张和张。

Lung cancer is the most critical type of malignant tumor that threatens human health. Traditional preclinical models have certain defects; for example, they cannot accurately reflect the characteristics of lung cancer and their development is costly and time-consuming. Through self-organization, cancer stem cells (CSCs) generate cancer organoids that have a structure similar to that of lung cancer tissues, overcoming to some extent the aforementioned challenges, thus enabling them to have broader application prospects. Lung cancer organoid (LCO) development methods can be divided into three broad categories based on the source of cells, which include cell lines, patient-derived xenografts and patient tumor tissue/pleural effusion. There are 17 different methods that have been described for the development of LCOs. These methods can be further merged into six categories based on the source of cells, the pre-treatment method used, the composition of the medium and the culture scaffold. These categories are: i) CSCs induced by defined transcription factors; ii) suspension culture; iii) relative optimal culture medium; iv) suboptimal culture medium; v) mechanical digestion and suboptimal culture medium; and vi) hydrogel scaffold. In the current review, the advantages and disadvantages of each of the aforementioned methods are summarized, and references for supporting studies are cited.Copyright: © 2024 Zhang and Zhang.