乳腺癌是全世界女性中最常见的恶性肿瘤之一，其发病率不断上升。体力活动，尤其是运动，已成为癌症预后的重要调节因素，影响肿瘤生物学和患者预后。在这项研究中，我们利用小鼠乳腺癌模型，将小鼠分为对照组和运动组；后者经历了21天的自愿运行。我们进行了RNA测序、生物信息学分析、泛癌分析和细胞实验，以研究运动影响的潜在机制。运动导致肿瘤大小和重量显着减小。运动后 mRNA 测序表明，运动小鼠中 THSD7B 显着上调，在癌症中的 MicroRNA 和钙信号通路等通路中观察到显着变化。在更广泛的癌症背景下，THSD7B 显示出相当大的表达变异性，在多种癌症中显着下调，与 PRAD、LAML、KIRC 和 GBM 的积极预后结果相关，并强调其作为预后标志物和治疗靶点的潜在作用。 THSD7B 表达还与乳腺癌细胞增殖、迁移和侵袭过程呈负相关。这项研究强调了运动在调节与肿瘤生长相关的基因表达方面的双重作用，并强调了 THSD7B 作为癌症治疗靶点的潜力。未来的研究应进一步探索运动和 THSD7B 影响癌症进展的具体机制，并开发免疫治疗增强策略来改变临床环境中的患者预后。版权所有 © 2024 Luo, Zhu, Fang, Xu, Wan, He, Chen, Chen, Wang 、刘和陈。

Breast cancer, one of the most prevalent malignancies among women worldwide, has rising incidence rates. Physical activity, particularly exercise, has emerged as a significant modifier of cancer prognosis, influencing both tumor biology and patient outcomes.In this study, we utilized a murine breast cancer model, dividing mice into a control group and an exercise group; the latter underwent 21 days of voluntary running. We conducted RNA sequencing, bioinformatics analysis, pan-cancer analysis, and cellular experiments to investigate the underlying mechanisms influenced by exercise.Exercise led to a significant reduction in tumor size and weight. Post-exercise mRNA sequencing indicated a notable upregulation of THSD7B in the exercised mice, with significant alterations observed in pathways such as MicroRNAs in cancers and the Calcium signaling pathway. In a broader cancer context, THSD7B showed considerable expression variability, being significantly downregulated in several cancers, correlating with positive prognostic outcomes in PRAD, LAML, KIRC, and GBM and highlighting its potential role as a prognostic marker and therapeutic target. THSD7B expression was also negatively associated with processes of breast cancer cell proliferation, migration, and invasion.This study underscores the dual role of exercise in modulating gene expression relevant to tumor growth and highlights the potential of THSD7B as a therapeutic target in cancer. Future research should further explore the specific mechanisms by which exercise and THSD7B influence cancer progression and develop immunotherapy-enhanced strategies to change patient outcomes in clinical settings.Copyright © 2024 Luo, Zhu, Fang, Xu, Wan, He, Chen, Chen, Wang, Liu and Chen.