2,3-二甲基喹喔啉 (DMQ) 是一种广谱抗菌植物化学物质。本研究旨在评估其毒理学特征。在适当的细胞培养物中进行的体外研究包括心脏毒性、肾毒性和肝毒性的评估。在小鼠中进行了一项体内研究以确定急性口服毒性（AOT）和亚急性口服毒性（SAOT）。在大鼠中进行了急性皮肤毒性（ADT）。 DMQ 的所有体外毒性研究在浓度≤100 μM 时均呈阴性结果，但人肝细胞癌细胞培养物中 ATP 没有显着降低。 DMQ的半数致死剂量高于2000mg/kg。所有动物均在预定的尸检中存活下来，并且没有任何动物表现出临床症状的任何改变。生化分析显示卫星组和对照组之间存在显着差异，血小板计数和白细胞计数分别增加了 99.8% 和 188.8%。组织学显示肾小体增大；睾酮分泌细胞增生；以及冠状动脉和毛细血管的扩张。目前的数据表明 DMQ 在啮齿动物中的安全性是可接受的，除了血小板增多、白细胞增多和高剂量的组织学变化需要进一步研究。版权所有：© 2024 Alfadil 等人。这是一篇根据知识共享署名许可条款分发的开放获取文章，允许在任何媒体上不受限制地使用、分发和复制，前提是注明原始作者和来源。

2,3-dimethylquinoxaline (DMQ) is a broad-spectrum antimicrobial phytochemical. This study aims to assess its toxicological profile. In vitro studies conducted in appropriate cell cultures, included assessment of cardiotoxicity, nephrotoxicity, and hepatotoxicity. An in vivo study was conducted in mice to determine acute oral toxicity (AOT), and subacute oral toxicity (SAOT). Acute dermal toxicity (ADT) was conducted in rats. All in-vitro toxicity studies of DMQ had negative results at concentrations ≤100 μM except for a non-significant reduction in the ATP in human hepatocellular carcinoma cell culture. The median lethal dose of DMQ was higher than 2000 mg/kg. All animals survived the scheduled necropsy and none showed any alteration in clinical signs. Biochemistry analysis revealed a significant difference between the satellite and control groups, showing an increase in platelet counts and white blood cell counts by 99.8% and 188.8%, respectively. Histology revealed enlargement of renal corpuscles; hyperplasia of testosterone-secreting cells; and dilatation of coronaries and capillaries. The present data suggests an acceptable safety profile of DMQ in rodents except for thrombocytosis, leukocytosis, and histological changes in high doses that need further investigation.Copyright: © 2024 Alfadil et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.