本研究旨在确定酵母菌后生元对猪空肠粘膜细胞更新、免疫反应和氧化应激的影响。 32 头体重为 6.05±0.24 公斤的新断奶猪根据随机完全区组设计被分配到两种饮食治疗组。治疗组为接受基础饮食的对照组，以及在基础饮食中添加酵母后生元（175 克/吨饮食）的一组。研究35天后，对猪实施安乐死，收集空肠粘膜以评估免疫状态、氧化应激、屏障标志物、细胞增殖和凋亡。酵母菌后生元从第3天到第7天降低了（P < 0.05）粪便评分，并倾向于增加干扰素-γ（IFN-γ）（P = 0.071）和雷帕霉素哺乳动物/机械靶标（mTOR）的基因表达（ P = 0.080），降低B细胞淋巴瘤2相关X蛋白1（BAX1）的基因表达（P < 0.05），倾向于降低血清和糖皮质激素诱导的蛋白激酶1（SGK1）的基因表达（P = 0.066），隐窝细胞增殖增加（P < 0.05），并倾向于增加空肠绒毛高度（P = 0.078）和隐窝深度（P = 0.052）。总之，在保育猪日粮中添加酵母菌后生元可减少断奶后第一周内的腹泻，并通过增强保育猪的免疫反应、促进隐窝细胞增殖和减少相关基因的表达来保护空肠绒毛。与细胞凋亡而不影响保育猪空肠的炎症和氧化应激状态。© 2024。作者。

This study aimed to determine the effects of Saccharomyces yeast postbiotics on cell turnover, immune responses, and oxidative stress in the jejunal mucosa of pigs. Thirty-two newly weaned pigs at 6.05 ± 0.24 kg were assigned to two dietary treatments based on a randomized complete block design. The treatments were control group receiving a basal diet and a group supplemented with Saccharomyces yeast postbiotics (175 g/ton diet) in the basal diet. After 35 d of the study, pigs were euthanized and jejunal mucosa were collected to assess immune status, oxidative stress, barrier markers, cell proliferation, and apoptosis. Saccharomyces yeast postbiotics reduced (P < 0.05) the fecal score from d 3 to d 7 and tended to increase the gene expression of interferon-γ (IFN-γ) (P = 0.071) and mammalian/mechanistic target of rapamycin (mTOR) (P = 0.080), decrease the gene expression of B-cell lymphoma 2-associated X protein 1 (BAX1) (P < 0.05), tended to decrease the gene expression of serum and glucocorticoid-induced protein kinase 1 (SGK1) (P = 0.066), increased (P < 0.05) cell proliferation in the crypts, and tended to increase the villus height (P = 0.078) and crypt depth (P = 0.052) in the jejunum. In conclusion, the supplementation of Saccharomyces yeast postbiotics in nursery diets reduced diarrhea within the first week after weaning and provided protection to the villi in the jejunum by enhancing the immune responses of nursery pigs, promoting crypt cell proliferation, and reducing the expression of genes associated with apoptosis without affecting inflammatory and oxidative stress status in the jejunum of the nursery pigs.© 2024. The Author(s).