硒纳米颗粒（SeNPs）作为一种潜在的癌症治疗剂，由于其高抗癌活性和低毒性而受到广泛关注。多糖可以作为改性剂和稳定剂，提高SeNPs在水溶液中的稳定性和分散性。本研究旨在研究从夏枯草簇中提取的异木聚糖 PVP3-1 稳定的 SeNP 的理化特性、稳定性和抗胰腺癌细胞活性。结果表明，分子量为154kDa的PVP3-1由​​→4)-β-D-Xylp(1→, →2, 4)-β-D-Xylp(1→, t-α-L-Araf组成利用多糖PVP3-1成功构建了(1→和4-MeO-α-D-GlcpA(1→)。红色、零价、均匀的球形SeNPs，平均直径约为60nm，在水溶液中稳定性高。抗胰腺癌细胞活性测定表明，PVP3-1-SeNPs 可以在体外抑制胰腺癌细胞的增殖和迁移，此外，PVP3-1-SeNPs 通过抑制 mTOR 信号通路诱导胰腺癌细胞凋亡和自噬。 ，这些结果表明 PVP3-1-SeNPs 可能是治疗胰腺癌的潜在抗肿瘤纳米颗粒。版权所有 © 2024。由 Elsevier B.V. 出版。

Selenium nanoparticles (SeNPs), as a potential cancer therapeutic agent, have attracted extensive attention due to their high anticancer activity and low toxicity. Polysaccharides could be the modifiers and stabilizers to improve the stability and dispersibility of SeNPs in aqueous solution. This study aimed to investigate the physicochemical characterization, stability, and anti-pancreatic cancer cell activities of SeNPs stabilized by a heteroxylan PVP3-1 extracted from the clusters of Prunella vulgaris Linn. Our results showed that PVP3-1 with Mw of 154 kDa was composed of →4)-β-D-Xylp(1→, →2, 4)-β-D-Xylp(1→, t-α-L-Araf(1→ and 4-MeO-α-D-GlcpA(1→. Red, zero-valent, and uniform spherical SeNPs with an average diameter of about 60 nm and high stability in aqueous solution were constructed successfully by polysaccharide PVP3-1. Anti-pancreatic cancer cell activity assays showed that PVP3-1-SeNPs could inhibit the proliferation and migration of pancreatic cancer cells in vitro. Furthermore, PVP3-1-SeNPs induced apoptosis and autophagy of pancreatic cancer cells through inhibiting mTOR signaling pathway. In conclusion, these results indicated that PVP3-1-SeNPs could be potential anti-tumor nanoparticles for treating pancreatic cancer.Copyright © 2024. Published by Elsevier B.V.