多糖的潜在抗肿瘤功能已被广泛接受，但多糖是否对CD8 T淋巴细胞攻击肿瘤细胞具有免疫调节作用尚不清楚。为了评估多糖化合物的CD8 T功能增强作用，建立了MC38-N4/OT-I共培养体系。富含α-吡喃葡萄糖的复合多糖的协同和互补免疫作用可以通过操纵抗原特异性T细胞扩增能力和功效来实现。本研究旨在通过测定共培养系统中 CD8 T 细胞的激活情况来研究富含 α-吡喃葡萄糖的复合多糖的抗肿瘤增强活性。与对照组（42.5%±0.72%）相比，富含α-吡喃葡萄糖的特定复合多糖（包括姬松茸、灰树花和陈皮）的含量显着下降（20.4%±1.23%，p<0.05）共培养系统中 MC38-N4 细胞的存活率。此外，富含α-吡喃葡萄糖的复合多糖导致CD8 T细胞和CD62L中央记忆T细胞的比例大幅增加（p<0.01），CD8 T细胞和CD62L中央记忆T细胞是分化程度较低的T细胞亚群，具有高免疫活性。总的来说，我们报道了特定的多糖组合，它重塑了细胞毒性T细胞的功能，并为使用特定类型的多糖改善免疫功能提供了基础。版权所有©2024。由Elsevier B.V.出版。

The potential antitumor function of polysaccharides is well accepted, it is unclear whether polysaccharides have immunoregulatory effect on CD8+ T lymphocyte cells to attack tumor cells. To evaluate the CD8+ T function enhancing role of polysaccharide compounds, the MC38-N4/OT-I co-culture system was established. The synergistic and complementary immune effect of α-glucopyranose-rich compound polysaccharides can be achieved by manipulating the antigen-specific T-cell expansion capacity and efficacy. This study was designed to investigate the antitumor-enhancement activity of a α-glucopyranose-rich compound polysaccharides by determining the activation of CD8+ T cells in a co-culture system. Compared to the control group (42.5 % ± 0.72 %), the specific α-glucopyranose-rich compound polysaccharides, comprising Agaricus blazei Murill, Grifola frondosa and Pericarpium Citri Reticulatae, demonstrated a significant decrease (20.4 % ± 1.23 %, p < 0.05) in the survival rate of MC38-N4 cells in the co-culture system. Additionally, the α-glucopyranose-rich compound polysaccharides resulted in a substantial increase (p < 0.01) in the proportion of CD8+ T cells and CD62L+ central memory T cells, which is a less differentiated T cell subset with high immune activity. Collectively, we reported that specific polysaccharide combination, which remodel the function of cytotoxic T cells and provided a basis for improving immune functions by using the specific types of polysaccharides.Copyright © 2024. Published by Elsevier B.V.