阿霉素 (DOX) 是一种临床用于治疗癌症的化疗药物，但其可能危及生命的心脏毒性作用仍然令人担忧。此后，本研究评估了 Lagenaria siceraria (LSS) 油对 DOX 诱发的大鼠心肌病的心脏保护作用将Wistar雄性大鼠(n=28，体重190-210g)任意分配成四个相等的组。第1组对照组（CTR）口服生理盐水（1ml/kg）；第 2 组 (DOX) 接受 DOX (10 mg/kg)；第3组（DOLS）接受DOX 3g Lagenaria siceraria种子油/kg；第 4 组 (LSSO) 每天接受 LSSO (3 g/kg)，持续 18 天。收集血清样本以确定肌酸激酶-MB (CK-MB) 同工酶、乳酸脱氢酶 (LDH)、天冬氨酸转氨酶 (AST) 和肌钙蛋白 I 活性。同时，对心脏组织中的过氧化氢酶、丙二醛（MDA）和还原型谷胱甘肽（GSH）进行了评估。此外，还对心脏组织进行了组织病理学研究。结果显示，与 CTR 组相比，DOLS 组的 CK-MB 水平没有显着变化（p > 0.05）。与 DOLS 和 LLSO 组相比，DOX 组证实 AST、LDH 和肌钙蛋白 1 血清水平显着增加 (p < 0.05)。该研究证明了 LSS 油对 DOX 引起的毒性的抗氧化活性。与 DOLS 和 LLSO 组相比，DOX 组显着降低了 GSH 和过氧化氢酶水平，同时增加了 MDA 水平。组织病理学分析显示 LSS 油对 DOX 引起的心肌损伤有保护作用。这项研究强调了 LSS 油在减轻大鼠模型中 DOX 引发的心脏毒性方面的有利影响。

Doxorubicin (DOX) is a chemotherapeutic drug applied clinically for the remedy of cancer, but its possibly life-threatening cardiotoxicity effects remain a concern.After that, this study evaluates the cardioprotective impacts of Lagenaria siceraria (LSS) oil on DOX induced cardiomyopathy in rats.Wistar male rats (n = 28, weighting 190-210 g) were arbitrarily allocated into four equal groups. Group 1 control group (CTR) received normal saline orally (1 ml/kg); group 2 (DOX) received DOX (10 mg/kg); group 3 (DOLS) received DOX + 3 g of Lagenaria siceraria seeds oil/kg; group 4 (LSSO) received LSSO (3 g/kg) daily for 18 days. The serum samples were collected to determine the creatine kinase-MB (CK-MB) isoenzyme, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), and Troponin I activity. At the same time, the catalase, malondialdehyde (MDA), and reduced glutathione (GSH) were assessed in heart tissues. Additionally, histopathological investigations for the heart tissue were performed.Results revealed no significant change in CK-MB levels between the DOLS group compared to the CTR group (p > 0.05). DOX group confirmed a substantial increase in AST, LDH, and Troponin1 serum levels compared to DOLS and LLSO groups (p < 0.05). The study demonstrated the antioxidant activity of LSS oil against DOX-induced toxicity. The DOX group significantly reduced GSH and catalase levels, with an increase in MDA levels compared to DOLS and LLSO groups. Histopathological analysis showed protective properties of LSS oil against myocardial damage caused by DOX.This study highlights the favorable impacts of LSS oil in mitigating DOX-triggered cardiotoxicity in a rat model.