旨在评估导管原位癌 (DCIS) 样本中的肿瘤浸润淋巴细胞 (TIL) 与疾病复发之间的关联。这项回顾性队列研究纳入了 2007 年 1 月至 2020 年 12 月期间接受治疗的 18 岁及以上女性。男性患者、个人根据手术标本的解剖病理学检查诊断为侵袭性或微侵袭性疾病的患者以及有任何其他癌症个人史的患者被排除在外。此外，还评估了“接触 TIL”（与肿瘤细胞直接接触的淋巴细胞）和导管周围结缔组织形成的存在，作为代表免疫微环境的补充方法。主要结果是基于 TIL 定量并针对潜在混杂因素进行调整的无复发生存率。病理学家评估了样本中肿瘤代表性最高的 TIL，并将其量化为百分比。使用 Kaplan-Meier 曲线、对数秩检验和 Cox 回归模型评估生存率。共有 191 名患者符合资格标准。平均随访时间为 77.2 个月，复发率为 9.2%。 TILs ≥17%的患者复发风险较高（HR 2.97，95% CI 1.17-7.51；p = 0.02）。此外，局灶性坏死（HR 6.4，95% CI 1.39-34.71；p = 0.018）或粉刺坏死（HR 4.53，95% CI 1.34-15.28；p = 0.015）与复发风险增加相关。根据多变量模型，粉刺坏死和 TILs ≥17% 与复发显着相关（分别为 p = 0.034 和 p = 0.035）。关于“触及 TIL”和导管周围结缔组织增生的评估，在评估其与疾病复发的关联时未发现统计学显着性。在我们的队列中，高百分比的 TIL（≥17%）和粉刺坏死的存在与 DCIS 复发独立相关.© 2024。作者获得 Springer Science Business Media, LLC（Springer Nature 的一部分）的独家许可。

To assess the association between tumor-infiltrating lymphocytes (TILs) in ductal carcinoma in situ (DCIS) samples and disease recurrence.This retrospective cohort study included women aged 18 years and older who underwent treatment between January 2007 and December 2020. Male patients, individuals diagnosed with invasive or microinvasive disease based on anatomopathological examination of surgical specimens, and those with a personal history of any other cancers were excluded. Additionally, the presence of "touching TILs" (lymphocytes in direct contact with tumor cells) and periductal desmoplasia were evaluated as complementary methods to represent the immunological microenvironment. The primary outcome was relapse-free survival based on TIL quantification adjusted for potential confounders. Pathologists assessed TILs in the sample with the highest tumor representation and quantified them as a percentage. Survival was evaluated using Kaplan‒Meier curves, log-rank tests, and Cox regression models.A total of 191 patients met the eligibility criteria. The mean follow-up duration was 77.2 months, with a recurrence rate of 9.2%. Patients with TILs ≥ 17% had a greater risk of recurrence (HR 2.97, 95% CI 1.17-7.51; p = 0.02). Additionally, focal necrosis (HR 6.4, 95% CI 1.39-34.71; p = 0.018) or comedonecrosis (HR 4.53, 95% CI 1.34-15.28; p = 0.015) were associated with increased recurrence risk. According to the multivariate model, comedonecrosis and TILs ≥ 17% were significantly associated with recurrence (p = 0.034 and p = 0.035, respectively). Regarding the evaluations of "touching TILs" and periductal desmoplasia, no statistical significance was found when assessing their association with disease recurrence.In our cohort, a high percentage of TILs (≥ 17%) and the presence of comedonecrosis were independently associated with DCIS recurrence.© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.