含有介孔SiO2-HA颗粒的三维壳聚糖/羟基磷灰石支架：促骨修复的全新步骤
3D chitosan/hydroxyapatite scaffolds containing mesoporous SiO2-HA particles: A new step to healing bone defects
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影响因子:8.5
分区:生物学2区 Top / 生化与分子生物学2区 应用化学2区 高分子科学2区
发表日期:2024 Oct
作者:
Nesa Abdian, Hamid Soltani Zangbar, Mohamadreza Etminanfar, Hamed Hamishehkar
DOI:
10.1016/j.ijbiomac.2024.135014
摘要
具有高机械强度且含有可降解成分的生物相容性支架，有望促进骨再生，优于非降解性骨修复材料。本研究通过冷冻干燥法制备了含有介孔二氧化硅-羟基磷灰石（SiO2-HA）颗粒的多孔壳聚糖（CS）/羟基磷灰石（HA）支架。扫描电子显微镜（FESEM）显示，将介孔SiO2-HA颗粒引入CS/HA支架，形成均匀的多孔结构，孔径从320 ± 1.1 μm减小到145 ± 1.4 μm。该支架的压缩强度为25 ± 1.2 MPa。在体外实验中，SAOS-2骨肉瘤成骨细胞系在支架上表现出良好的粘附、铺展和增殖，表明支架提供了适宜的细胞培养环境。此外，在大鼠植入缺损部位的体内分析证实，含有介孔SiO2-HA颗粒的CS/HA支架能促进骨再生，通过增强骨导性和上调成骨基因表达（提高至19.31，即比对照组高约5倍）来促进骨形成。在手术后21天，支架中的新骨形成比例达90 %。因此，将介孔SiO2-HA颗粒引入CS/HA支架，为未来的组织工程和再生医学提供了新的策略。
Abstract
Biocompatible scaffolds with high mechanical strengths that contain biodegradable components could boost bone regeneration compared with nondegradable bone repair materials. In this study, porous chitosan (CS)/hydroxyapatite (HA) scaffolds containing mesoporous SiO2-HA particles were fabricated through the freeze-drying process. According to field emission scanning electron microscopy (FESEM) results, combining mesoporous SiO2-HA particles in CS/HA scaffolds led to a uniform porous structure. It decreased pore sizes from 320 ± 1.1 μm to 145 ± 1.4 μm. Moreover, the compressive strength value of this scaffold was 25 ± 1.2 MPa. The in-vitro approaches exhibited good sarcoma osteogenic cell line (SAOS-2) adhesion, spreading, and proliferation, indicating that the scaffolds provided a suitable environment for cell cultivation. Also, in-vivo analyses in implanted defect sites of rats proved that the CS/HA/mesoporous SiO2-HA scaffolds could promote bone regeneration via enhancing osteoconduction and meliorating the expression of osteogenesis gene to 19.31 (about 5-fold higher compared to the control group) by exposing them to the bone-like precursors. Further, this scaffold's new bone formation percentage was equal to 90 % after 21 days post-surgery. Therefore, incorporating mesoporous SiO2-HA particles into CS/HA scaffolds can suggest a new future tissue engineering and regeneration strategy.