与不可降解的骨修复材料相比，含有可生物降解成分的具有高机械强度的生物相容性支架可以促进骨再生。在这项研究中，通过冷冻干燥过程制备了含有介孔SiO2-HA颗粒的多孔壳聚糖（CS）/羟基磷灰石（HA）支架。根据场发射扫描电子显微镜 (FESEM) 结果，在 CS/HA 支架中结合介孔 SiO2-HA 颗粒可形成均匀的多孔结构。孔径从320±1.1μm减小到145±1.4μm。另外，该支架的抗压强度值为25±1.2MPa。体外方法表现出良好的肉瘤成骨细胞系（SAOS-2）粘附、扩散和增殖，表明支架为细胞培养提供了合适的环境。此外，对大鼠植入缺损部位的体内分析证明，CS/HA/介孔SiO2-HA支架可以通过增强骨传导和改善成骨基因的表达来促进骨再生至19.31（比对照高约5倍）组）通过将它们暴露于类骨前体中。此外，术后21天后，该支架的新骨形成率达到90%。因此，将介孔 SiO2-HA 颗粒纳入 CS/HA 支架中可以提出一种新的未来组织工程和再生策略。版权所有 © 2024。由 Elsevier B.V. 出版。

Biocompatible scaffolds with high mechanical strengths that contain biodegradable components could boost bone regeneration compared with nondegradable bone repair materials. In this study, porous chitosan (CS)/hydroxyapatite (HA) scaffolds containing mesoporous SiO2-HA particles were fabricated through the freeze-drying process. According to field emission scanning electron microscopy (FESEM) results, combining mesoporous SiO2-HA particles in CS/HA scaffolds led to a uniform porous structure. It decreased pore sizes from 320 ± 1.1 μm to 145 ± 1.4 μm. Moreover, the compressive strength value of this scaffold was 25 ± 1.2 MPa. The in-vitro approaches exhibited good sarcoma osteogenic cell line (SAOS-2) adhesion, spreading, and proliferation, indicating that the scaffolds provided a suitable environment for cell cultivation. Also, in-vivo analyses in implanted defect sites of rats proved that the CS/HA/mesoporous SiO2-HA scaffolds could promote bone regeneration via enhancing osteoconduction and meliorating the expression of osteogenesis gene to 19.31 (about 5-fold higher compared to the control group) by exposing them to the bone-like precursors. Further, this scaffold's new bone formation percentage was equal to 90 % after 21 days post-surgery. Therefore, incorporating mesoporous SiO2-HA particles into CS/HA scaffolds can suggest a new future tissue engineering and regeneration strategy.Copyright © 2024. Published by Elsevier B.V.