TBC1D4激活蛋白质分子的表达特征，并鉴定了预防甲状腺癌进展的关键模块基因
Expression characteristics of TBC1D4 activating protein molecule and identification of key module genes for preventing thyroid cancer progression
影响因子:8.50000
分区:生物学2区 Top / 生化与分子生物学2区 应用化学2区 高分子科学2区
发表日期:2024 Oct
作者:
Na Wu, Zuoqian Jing, Huina Lv, Qun Liu, Ming Gu, Yifan Zhong, Peng Xing, Ruiyang Ma, Yuchen Jing
摘要
甲状腺癌等内分泌肿瘤变得越来越频繁。找不到临床信息丰富的预测因素。因此，有效的基因网络和代表性的生物标志物可以照亮甲状腺癌预防分子机制。 TBC1D4是一种激活的蛋白质分子，在调节细胞代谢和信号转导中起重要作用。这项研究的目的是研究TBC1D4激活蛋白质分子的表达特征，并确定预防甲状腺癌进展的关键模块基因。 GSE65144数据从GEO下载。 r中的“ limma”找到了错误的发现率<0.05，log2倍变化<1。 WGCNA建立基因共表达网络，屏幕键模块和过滤器基因。重叠基因成为集线器基因。集线器基因进行了GO和KEGG途径富集分析。我们使用套索提取集线基因表达结果的独特基因。关键基因。 GEPIA数据库确定了表达和生存影响。总共3220摄氏度。甲状腺癌主要与深色，深色图犬和绿色模块有关。维恩筛选了639个集线器基因。细胞因子 - 环氨酸受体相互作用是主要的KEGG富集。集线器基因为14。最后，Arhgap6，TBC1D4和TC2N是重要的基因。通过基因筛选和功能富集分析，我们确定了与TBC1D4激活蛋白质相关的一组基因，并构建了相应的蛋白质相互作用网络。
Abstract
Endocrine tumors like thyroid carcinoma are becoming more frequent. No clinically informative predictors were found. Thus, effective gene networks and representative biomarkers can illuminate thyroid cancer prevention molecular mechanisms. TBC1D4 is an activating protein molecule that plays an important role in regulating cell metabolism and signal transduction. The aim of this study was to investigate the expression characteristics of TBC1D4 activating protein molecules and identify key module genes that prevent thyroid cancer progression. GSE65144 data were downloaded from GEO. "limma" in R found DEGs with a false discovery rate < 0.05 and a log2 fold change <1. WGCNA builds gene co-expression networks, screens key modules, and filters hub genes. Overlapping genes become hub genes. Hub genes underwent GO and KEGG pathway enrichment analysis. We used Lasso to extract hub gene expression results' distinctive genes. Key genes. GEPIA database determined expression and survival impact. A total of 3220 DEGs. Thyroid cancer was mostly associated with darkred, darkturquoise, and green modules. Venn screened 639 hub genes. Cytokine-cytokine receptor interaction was the primary KEGG enrichment. Hub genes were 14. Finally, ARHGAP6, TBC1D4, and TC2N were important genes. Through gene screening and functional enrichment analysis, we identified a group of genes related to TBC1D4 activating protein and constructed the corresponding protein interaction network.