甲状腺癌等内分泌肿瘤变得越来越常见。没有发现临床信息预测因子。因此，有效的基因网络和代表性生物标志物可以阐明甲状腺癌预防的分子机制。 TBC1D4是一种激活蛋白分子，在调节细胞代谢和信号转导中发挥重要作用。本研究的目的是研究TBC1D4激活蛋白分子的表达特征并鉴定预防甲状腺癌进展的关键模块基因。 GSE65144 数据是从 GEO 下载的。 R 中的“limma”发现错误发现率 < 0.05 且 log2 倍数变化 <1 的 DEG。 WGCNA构建基因共表达网络，筛选关键模块，过滤枢纽基因。重叠的基因成为中心基因。 Hub基因进行GO和KEGG通路富集分析。我们使用Lasso提取中心基因表达结果的独特基因。关键基因。 GEPIA 数据库确定了表达和生存影响。总共3220°。甲状腺癌主要与深红色、深绿松石色和绿色模块有关。维恩筛选了 639 个枢纽基因。细胞因子-细胞因子受体相互作用是主要的 KEGG 富集。 Hub基因有14个。最后，ARHGAP6、TBC1D4和TC2N是重要的基因。通过基因筛选和功能富集分析，我们鉴定了一组与TBC1D4激活蛋白相关的基因，并构建了相应的蛋白相互作用网络。版权所有©2024 Elsevier B.V.保留所有权利。

Endocrine tumors like thyroid carcinoma are becoming more frequent. No clinically informative predictors were found. Thus, effective gene networks and representative biomarkers can illuminate thyroid cancer prevention molecular mechanisms. TBC1D4 is an activating protein molecule that plays an important role in regulating cell metabolism and signal transduction. The aim of this study was to investigate the expression characteristics of TBC1D4 activating protein molecules and identify key module genes that prevent thyroid cancer progression. GSE65144 data were downloaded from GEO. "limma" in R found DEGs with a false discovery rate < 0.05 and a log2 fold change <1. WGCNA builds gene co-expression networks, screens key modules, and filters hub genes. Overlapping genes become hub genes. Hub genes underwent GO and KEGG pathway enrichment analysis. We used Lasso to extract hub gene expression results' distinctive genes. Key genes. GEPIA database determined expression and survival impact. A total of 3220 DEGs. Thyroid cancer was mostly associated with darkred, darkturquoise, and green modules. Venn screened 639 hub genes. Cytokine-cytokine receptor interaction was the primary KEGG enrichment. Hub genes were 14. Finally, ARHGAP6, TBC1D4, and TC2N were important genes. Through gene screening and functional enrichment analysis, we identified a group of genes related to TBC1D4 activating protein and constructed the corresponding protein interaction network.Copyright © 2024 Elsevier B.V. All rights reserved.