将硒纳米粒子（SeNPs）与生物活性多糖结合是克服SeNPs和多糖的缺点并获得新型抗肿瘤候选药物的有效方法之一。在这项研究中，从 Marsdenia tenacissima (Roxb.) Wight et Arn. 的茎中分离出一种具有中等抗肝癌活性的杂多糖 (MTP70)。为了进一步提高MTP70的抗肝癌活性和SeNPs的应用，设计并制备了一种新型稳定的纳米粒子（MTP-SeNP）。 MTP-SeNPs（Se含量为8.25%）为单分散球形纳米粒子（50nm），MTP70通过CO⋯Se键的形成包裹在SeNPs表面，在水中具有高稳定性和良好的分散性近一个月。此外，与MTP70相比，MTP-SeNPs表现出更高的抑制效果。 MTP-SeNPs可通过诱导细胞凋亡、将细胞周期阻滞于S期，有效抑制HePG2细胞的增殖、侵袭和转移，这与Bax/Bcl-2/Caspase和p-21/的激活密切相关。 Akt/Cyclin A2 信号通路。我们的研究结果为M. tenacissima多糖的进一步开发和应用提供了理论基础，并表明MTP-SeNPs可以在制药和生物医学行业中探索作为一种有前途的抗肝癌药物。版权所有©2024。由Elsevier B.V.出版。

Combining selenium nanoparticles (SeNPs) with bioactive polysaccharides is one of the effective ways to overcome the shortcomings of SeNPs and polysaccharide and obtain novel antitumor drug candidates. In this study, a heteropolysaccharide (MTP70) with moderate antihepatoma activity was isolated from the stems of Marsdenia tenacissima (Roxb.) Wight et Arn. To further improve the antihepatoma activity of MTP70 and the application of SeNPs, a novel stable nanoparticle (MTP-SeNP) was designed and fabricated. MTP-SeNPs (Se content of 8.25 %) were characterized as monodisperse spherical nanoparticles (50 nm) with MTP70 wrapped on the surface of the SeNPs by the formation of CO⋯Se bonds and possessed high stability and good dispersion in water for almost a month. In addition, MTP-SeNPs showed higher inhibitory effect compared with MTP70. MTP-SeNPs could effectively inhibit the proliferation, invasion, and metastasis of HePG2 cells by inducing apoptosis and arresting the cell cycle at the S phase, which were closely related to the activation of the Bax/Bcl-2/Caspases and p-21/Akt/Cyclin A2 signaling pathways. Our results provide a theoretical basis for further development and application of M. tenacissima polysaccharide, and show that MTP-SeNPs could be explored as a promising anti-hepatoma agent in the pharmaceutical and biomedical industries.Copyright © 2024. Published by Elsevier B.V.