黄曲霉毒素 B1 (AFB1) 是一种强效肝癌致癌物，通过诱导肝细胞 DNA 变化而致癌。基于单模式检测策略，已经开发了多种检测 AFB1 的方法。兼容 AFB1 多模态检测的新型平台的制造为 AFB1 的可靠检测提供了强大的性能。在本研究中，我们的目标是开发一个强大的生物传感平台，结合电化学和荧光技术，用于敏感和特异地检测黄曲霉毒素B1。该传感平台包括磁性核壳Fe3O4@AuNPs和沸石咪唑酯骨架-8（ZIF-8） ）。在电化学模式中，通过玻碳电极的功能化使用所应用的伏安法，并表现出 0.5 至 10000 pg mL-1 之间的线性范围，LOD 为 0.32 pg mL-1。荧光分析基于沉积在同一平台上的 FAM 功能化适体的 FRET 开/关状态。通过添加浓度范围为 6-60 fg mL-1 且 LOD 为 0.20 fg mL-1 的 AFB1 来恢复 FAM 发射。使用电化学方法和荧光方法对实际样品分析表明，相对回收率分别在92.81-105.32%和91.66-106.66%范围内，其可靠性得到了HPLC技术的证实。实验结果证实了所提出的适体传感器作为一个灵敏、高效、精确的平台，用于在电化学和荧光检测方法中监测 AFB1。所提出的策略显示出不同分析物之间的有效选择性并且具有可重复性。此外，生物传感器的适用性在食品和生物样品中得到了证实。版权所有 © 2024 Elsevier B.V. 保留所有权利。

Aflatoxin B1 (AFB1), is a potent hepatic carcinogen which causes cancer by inducing DNA changes in the liver cells. Variety of methods have been developed for detection of AFB1 which are based on single mode detection strategy. Fabrication of novel platform which are compatible for multimodal detection of AFB1 provide robust performance for reliable detection of AFB1. In this study, we aimed to develop a robust biosensing platform that combines electrochemical and fluorescence techniques for the sensitive and specific detection of Aflatoxin B1.The sensing platform includes the magnetic core-shell Fe3O4@AuNPs and zeolitic imidazolate framework-8 (ZIF-8). In electrochemical mode, the applied voltametric approach was used through functionalization of glassy carbon electrode and exhibited a linear range between 0.5 and 10000 pg mL-1 with LOD of 0.32 pg mL-1. Fluorescence analysis was based on the FRET on/off status of FAM-functionalized aptamer deposited on the same platform. The FAM emission recovered by the addition of AFB1 concentration in the range of 6-60 fg mL-1 with the LOD of 0.20 fg mL-1. The real sample analysis demonstrated satisfactory relative recoveries in the range of 92.81-105.32 % and 91.66-106.66 % using the electrochemical and fluorescence methods, respectively, and its reliability was confirmed by the HPLC technique.The experimental results affirm that the proposed aptasensor serves as a sensitive, efficient, and precise platform for monitoring AFB1 in both electrochemical and fluorescence detection approaches. Proposed strategy showed efficient selectivity among different analytes and was reproducible. Furthermore, the applicability of biosensor was confirmed in food and biological samples.Copyright © 2024 Elsevier B.V. All rights reserved.