转录因子 POU2F1 调节肿瘤中 microRNA 的表达水平。然而，胃癌（GC）中POU2F1调节的miR-29b1/a簇仍然未知。使用逆转录聚合酶链反应（PCR）、蛋白质印迹、免疫组织化学和RNA原位杂交评估GC细胞中的基因表达。进行免疫共沉淀以评估蛋白质相互作用。进行Transwell迁移和侵袭实验来研究GC细胞的生物学行为。在 GC 细胞中进行了 3'-UTR 研究的 MiR-29b1/a 簇启动子分析和荧光素酶活性测定。在裸鼠中评估体内肿瘤转移。POU2F1 在 GC 细胞系中过表达并与 miR-29b1/a 簇启动子结合。在 GC 组织中，POU2F1 上调，而成熟 miR-29b-3p 和 miR-29a-3p 下调。 POU2F1 通过抑制体外和体内 miR-29b-3p 或 miR-29a-3p 表达来促进 GC 转移。此外，PIK3R1和/或PIK3R3是miR-29b-3p和/或miR-29a-3p的直接靶标，并且PIK3R1或PIK3R3的异位表达逆转成熟miR-29b-3p和/或miR-29a的抑制作用-3p对GC细胞转移和侵袭的影响。此外，PIK3R1 与 PIK3R3 的相互作用促进迁移和侵袭，miR-29b-3p、miR-29a-3p、PIK3R1 和 PIK3R3 通过磷脂酰肌醇 3-激酶/蛋白激酶 B/雷帕霉素的哺乳动物靶标调节迁移和侵袭。 GC 细胞中的 PI3K/Akt/mTOR) 通路。此外，GC组织样本中POU2F1、PIK3R1和PIK3R3的表达水平与miR-29b-3p和miR-29a-3p的表达水平呈负相关。 POU2F1-miR-29b-3p/miR-29a-3p-PIK3R1/PIK3R1信号轴调节肿瘤进展，可能是 GC 的一个有前途的治疗靶点。版权所有 © 2024 中华医学会，由 Wolters Kluwer, Inc. 根据 CC-BY-NC-ND 许可制作。

The transcription factor POU2F1 regulates the expression levels of microRNAs in neoplasia. However, the miR-29b1/a cluster modulated by POU2F1 in gastric cancer (GC) remains unknown.Gene expression in GC cells was evaluated using reverse-transcription polymerase chain reaction (PCR), western blotting, immunohistochemistry, and RNA in situ hybridization. Co-immunoprecipitation was performed to evaluate protein interactions. Transwell migration and invasion assays were performed to investigate the biological behavior of GC cells. MiR-29b1/a cluster promoter analysis and luciferase activity assay for the 3'-UTR study were performed in GC cells. In vivo tumor metastasis was evaluated in nude mice.POU2F1 is overexpressed in GC cell lines and binds to the miR-29b1/a cluster promoter. POU2F1 is upregulated, whereas mature miR-29b-3p and miR-29a-3p are downregulated in GC tissues. POU2F1 promotes GC metastasis by inhibiting miR-29b-3p or miR-29a-3p expression in vitro and in vivo. Furthermore, PIK3R1 and/or PIK3R3 are direct targets of miR-29b-3p and/or miR-29a-3p, and the ectopic expression of PIK3R1 or PIK3R3 reverses the suppressive effect of mature miR-29b-3p and/or miR-29a-3p on GC cell metastasis and invasion. Additionally, the interaction of PIK3R1 with PIK3R3 promotes migration and invasion, and miR-29b-3p, miR-29a-3p, PIK3R1, and PIK3R3 regulate migration and invasion via the phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/Akt/mTOR) pathway in GC cells. In addition, POU2F1, PIK3R1, and PIK3R3 expression levels negatively correlated with miR-29b-3p and miR-29a-3p expression levels in GC tissue samples.The POU2F1-miR-29b-3p/miR-29a-3p-PIK3R1/PIK3R1 signaling axis regulates tumor progression and may be a promising therapeutic target for GC.Copyright © 2024 The Chinese Medical Association, produced by Wolters Kluwer, Inc. under the CC-BY-NC-ND license.