纳米粒子（NPs）在肿瘤区域的有效积累是当前纳米技术研究的重要目标，而靶向纳米平台是一种有效的解决方案。因此，我们设计了一种多功能声敏靶向纳米颗粒，它结合了声敏剂，可实现精确靶向、深穿透的声动力治疗 (SDT) 与多模态成像相结合，用于肾细胞癌 (RCC) 的诊断和监测。ZnPP@PP NPs (通过双乳液法制备ZnPP@PLGA-PFP NPs，并通过二亚胺碳法将G250共价连接到NPs壳上。对ZnPP@G-PP纳米粒子进行了理化性质测试，包括粒径、电位分布、包封率和载药能力测试。我们评估了纳米粒子的体外靶向能力、活性氧（ROS）的产生和渗透性。此外，我们还评估了纳米粒子的多模态成像能力以及体外和体内对肾细胞癌的治疗能力。成功构建了Znpp@G-PP NPs，其综合性能表现出粒径均匀、负电位和良好的稳定性。该纳米颗粒成功负载ZnPP并与G250连接，表现出肿瘤特异性靶向能力。在LIFU照射下，纳米颗粒通过SDT产生1O2。对于 RCC，Znpp@G-PP NP 的 PA/US 多模态成像可提供诊断信息并实时监测 786-O RCC 异种移植物的治疗情况，具有良好的生物相容性。通过UTMD，纳米粒子可以有效地靶向肿瘤细胞并渗透到肿瘤内部，显着提高SDT效果。体外和体内实验表明，纳米颗粒与LIFU联合可以抑制肿瘤，并通过免疫组织化学证实了治疗效果。ZnPP@G-PP NPs为肾细胞癌提供了一种有前途的治疗策略，并为进一步研究改善肾细胞癌提供了平台。诊断和治疗的功效。© 2024 Dai et al.

The effective accumulation of nanoparticles (NPs) in the tumour area is an important goals of current nanotechnology research, and a targeted nanoplatform is an effective solution. So we designed a multifunctional sound-sensitive targeted NP that combines a sonosensitizer to enable precisely targeted, deep-penetration sonodynamic therapy (SDT) in combination with multimodal imaging for the diagnosis and monitoring of renal cell carcinoma (RCC).ZnPP@PP NPs (ZnPP@PLGA- PFP NPs) were prepared via a double emulsion method, and G250 was covalently attached to the NPs shell via the carbon diimide method. Physicochemical property tests were conducted on the ZnPP@G-PP NPs, including tests of particle size, potential distribution, encapsulation efficiency and drug loading capability. We assessed the targeting ability, the production of reactive oxygen species (ROS) and permeability of the NPs in vitro. Moreover, we evaluated the nanoparticle's multimodal imaging capabilities and therapeutic ability against RCC, both in vitro and in vivo.The Znpp@G-PP NPs were successfully constructed, and their general properties showed uniform particle size, negative potential and good stability. The nanoparticles were successfully loaded with ZnPP and connected with G250, showing tumor-specific targeting ability. Under LIFU irradiation, the nanoparticles produced 1O2 by SDT. For RCC, PA/US multi-modal imaging of Znpp@G-PP NPs provide diagnostic information and monitor therapies in real time in 786-O RCC xenografts, with good biocompatibility. With the UTMD, nanoparticles can be effectively targeted into the tumor cells and penetrate into the tumor interior, significantly improving the SDT effect. Experiments in vitro and in vivo showed that the combination of the nanoparticles and LIFU could suppress the tumor, and the therapeutic effect was confirmed by immunohistochemistry.ZnPP@G-PP NPs provide a promising theranostic strategy for RCC and a platform for further research on improving the efficacy of diagnosis and treatment.© 2024 Dai et al.