联合使用UTMD纳米平台，可有效递送治疗肾细胞癌的药物
Combined UTMD-Nanoplatform for the Effective Delivery of Drugs to Treat Renal Cell Carcinoma
影响因子:6.50000
分区:医学2区 / 药学2区 纳米科技3区
发表日期:2024
作者:
Ting Dai, Qimeihui Wang, Lingyu Zhu, Qiang Luo, Jiayu Yang, Xia Meng, Hui Wang, Zhixia Sun
摘要
纳米颗粒（NP）在肿瘤区域的有效积累是当前纳米技术研究的重要目标，而靶向纳米植物是一个有效的解决方案。因此，我们设计了一种多功能声音靶向的NP，结合了超声敏化剂，以启用精确靶向的，深层的超声波动力学治疗（SDT），并结合多模态成像，以诊断和监测肾细胞癌（RCC）的诊断和监测。通过二酰亚胺碳方法共价连接到NPS壳。在ZNPP@G-PP NP上进行了物理化学性质测试，包括粒径，潜在分布，封装效率和药物加载能力的测试。我们评估了靶向能力，活性氧的产生（ROS）和NPS在体外的渗透性。此外，我们评估了纳米颗粒的多模式成像能力和针对RCC的治疗能力，无论是在体外还是体内。ZNPP@G-PPP NPS成功地构建了，其一般特性显示出均匀的粒径，负势，潜在的势和良好的稳定性。纳米颗粒成功加载了ZNPP并与G250相连，显示出肿瘤特异性的靶向能力。在LIFU照射下，SDT产生的纳米颗粒1O2。对于RCC，ZNPP@G-PP的PA/US多模式成像可在786-O RCC异种移植物中实时提供诊断信息和监测疗法，具有良好的生物相容性。使用UTMD，可以有效地将纳米颗粒靶向肿瘤细胞并渗透到肿瘤内部，从而显着改善SDT效应。体外和体内实验表明，纳米颗粒和LIFU的组合可以抑制肿瘤，并且通过免疫组织化学确认了治疗效应。znpp@g-ppp NPS提供了有希望的RCC的有希望的RCC的策略，并为改善诊断和治疗的效率提供了进一步研究的平台。
Abstract
The effective accumulation of nanoparticles (NPs) in the tumour area is an important goals of current nanotechnology research, and a targeted nanoplatform is an effective solution. So we designed a multifunctional sound-sensitive targeted NP that combines a sonosensitizer to enable precisely targeted, deep-penetration sonodynamic therapy (SDT) in combination with multimodal imaging for the diagnosis and monitoring of renal cell carcinoma (RCC).ZnPP@PP NPs (ZnPP@PLGA- PFP NPs) were prepared via a double emulsion method, and G250 was covalently attached to the NPs shell via the carbon diimide method. Physicochemical property tests were conducted on the ZnPP@G-PP NPs, including tests of particle size, potential distribution, encapsulation efficiency and drug loading capability. We assessed the targeting ability, the production of reactive oxygen species (ROS) and permeability of the NPs in vitro. Moreover, we evaluated the nanoparticle's multimodal imaging capabilities and therapeutic ability against RCC, both in vitro and in vivo.The Znpp@G-PP NPs were successfully constructed, and their general properties showed uniform particle size, negative potential and good stability. The nanoparticles were successfully loaded with ZnPP and connected with G250, showing tumor-specific targeting ability. Under LIFU irradiation, the nanoparticles produced 1O2 by SDT. For RCC, PA/US multi-modal imaging of Znpp@G-PP NPs provide diagnostic information and monitor therapies in real time in 786-O RCC xenografts, with good biocompatibility. With the UTMD, nanoparticles can be effectively targeted into the tumor cells and penetrate into the tumor interior, significantly improving the SDT effect. Experiments in vitro and in vivo showed that the combination of the nanoparticles and LIFU could suppress the tumor, and the therapeutic effect was confirmed by immunohistochemistry.ZnPP@G-PP NPs provide a promising theranostic strategy for RCC and a platform for further research on improving the efficacy of diagnosis and treatment.