人乳头瘤病毒（HPV）是宫颈癌的重要致病因素，并与非小细胞肺癌（NSCLC）相关。默克尔细胞多瘤病毒 (MCPyV) 是一种罕见且高度致命的皮肤病毒，可导致默克尔细胞癌 (MCC)。尽管同时感染致癌 HPV 和 MCPyV 可能会增加患癌症的风险，但明确的病因学联系尚未确定。最近，报道了不同种族群体中 MCPyV 非编码控制区（NCCR）的基因组变异和遗传多样性。本研究旨在提供 NSCLC 患者中 HPV 和 MCPyV 感染/合并感染的准确患病率信息，并评估和确认韩国 MCPyV NCCR 变异基因型和序列。通过针对大 T 抗原 (LT-ag)、病毒衣壳蛋白 1 (VP1) 和 NCCR 区域的聚合酶链反应 (PCR) 评估来自 150 个 NSCLC 组织和 150 个邻近对照组织的 DNA。在韩国患者的 22.7%（150 例中的​​ 34 例）NSCLC 组织和 8.0%（150 例中的​​ 12 例）癌旁组织中检测到 MCPyV。伴有和不伴有 MCPyV 的 HPV 发病率分别为 26.5%（34 例中的 9 例）和 12.9%（116 例中的 15 例）。韩国患者中 I 亚型 MCPyV NCCR 基因型患病率为 21.3%（150 人中的 32 人），IIc 亚型患病率为 6%（150 人中的 9 人）。 I 亚型是一种主要的东亚菌株，含有 25bp 串联重复序列，在 MCPyV NCCR 数据集中最常见。我们的结果证实，与其他肿瘤相关病毒的共感染与 NSCLC 无关。尽管 NCCR 重排在 MCPyV 感染中的作用仍不清楚，但未来的研究有必要确定 MCPyV NCCR 序列重排与特定疾病的关联。© 2024 Wiley periodicals LLC。

Human papillomavirus (HPV) is an important causative factor of cervical cancer and is associated with nonsmall cell lung cancer (NSCLC). Merkel cell polyomavirus (MCPyV) is a rare and highly fatal cutaneous virus that can cause Merkel cell carcinoma (MCC). Although coinfection with oncogenic HPV and MCPyV may increase cancer risk, a definitive etiological link has not been established. Recently, genomic variation and genetic diversity in the MCPyV noncoding control region (NCCR) among ethnic groups has been reported. The current study aimed to provide accurate prevalence information on HPV and MCPyV infection/coinfection in NSCLC patients and to evaluate and confirm Korean MCPyV NCCR variant genotypes and sequences. DNA from 150 NSCLC tissues and 150 adjacent control tissues was assessed via polymerase chain reaction (PCR) targeting regions of the large T antigen (LT-ag), viral capsid protein 1 (VP1), and NCCR. MCPyV was detected in 22.7% (34 of 150) of NSCLC tissues and 8.0% (12 of 150) of adjacent tissues from Korean patients. The incidence rates of HPV with and without MCPyV were 26.5% (nine of 34) and 12.9% (15 of 116). The MCPyV NCCR genotype prevalence in Korean patients was 21.3% (32 of 150) for subtype I and 6% (nine of 150) for subtype IIc. Subtype I, a predominant East Asian strain containing 25 bp tandem repeats, was most common in the MCPyV NCCR data set. Our results confirm that coinfection with other tumor-associated viruses is not associated with NSCLC. Although the role of NCCR rearrangements in MCPyV infection remains unknown, future studies are warranted to determine the associations of MCPyV NCCR sequence rearrangements with specific diseases.© 2024 Wiley Periodicals LLC.