MicroRNA (miRNA) 因其诊断能力而受到广泛关注，例如缺氧认知障碍和癌症。然而，现有的miRNA检测方法普遍因灵敏度低和干扰分子导致的假阳性检测等缺点而受到批评。在这里，我们通过结合基于目标识别的挂锁环化、基于固定引物的信号放大和个人血糖仪，提供了一种新颖、灵敏且便携式的 miRNA 检测方法。该方法具有多种优点，包括精确识别特定位点、卓越的灵敏度和无需仪器的特点。这些属性为癌症和缺氧认知障碍等各种疾病的诊断和临床研究带来了巨大希望，使人们能够更深入地了解其病理和生理方面。

MicroRNA (miRNA) has garnered considerable attention due to its diagnostic capabilities, such as in hypoxic cognitive impairment and cancers. However, the existing miRNA detection methods are commonly criticized for the drawbacks of low sensitivity and false-positive detection derived from interfering molecules. Here, we provide a novel, sensitive and portable method for miRNA detection by combining target identification based cyclization of padlocks, immobilized primer-based signal amplification and a personal glucose meter. The proposed method exhibits several advantages, including precise identification of specific sites, exceptional sensitivity and instrument-free feature. These attributes hold great promise for the diagnosis and clinical investigation of various diseases, such as cancer and hypoxic cognitive impairment, enabling a deeper understanding of their pathological and physiological aspects.