美金刚是一种 N-甲基-d-天冬氨酸受体拮抗剂，广泛用于治疗阿尔茨海默病，并被发现具有潜在的神经保护作用。在这项研究中，我们评估了美金刚对顺铂引起的耳毒性的保护作用。顺铂是一种广泛用于治疗多种癌症的抗癌药物。然而，其使用受到其副作用（包括耳毒性）的限制。已经开发了几种药物来降低顺铂的毒性。在本研究中，我们用美金刚治疗顺铂损伤的耳蜗毛细胞并评估其保护作用。House Ear Institute Corti 1 (HEI-OC1) 细胞和耳蜗外植体用顺铂或美金刚治疗。测量细胞活力、凋亡模式、活性氧 (ROS) 产生、Bcl-2/caspase-3 活性和细胞数量，以评估美金刚的抗凋亡和抗氧化作用。美金刚治疗显着提高细胞活力并减少顺铂 -诱导听觉细胞凋亡。 Bcl-2/caspase-3 活性也显着增加，表明对顺铂引起的耳毒性具有抗凋亡作用。我们的结果表明，美金刚在体外可防止顺铂引起的耳毒性，为预防接受化疗的患者听力损失提供了潜在的新策略顺铂化疗。版权所有 © 2024, Otology

Memantine, an N-methyl-d-aspartate receptor antagonist, is widely used to treat Alzheimer's disease and has been found to have potential neuroprotective effects. In this study, we evaluated the protective effects of memantine against cisplatin-induced ototoxicity.Cisplatin is a widely used anticancer drug for various cancers; however, its use is limited by its side effects, including ototoxicity. Several drugs have been developed to reduce cisplatin toxicity. In this study, we treated cisplatin-damaged cochlear hair cells with memantine and evaluated its protective effects.House Ear Institute Organ of Corti 1 (HEI-OC1) cells and cochlear explants were treated with cisplatin or memantine. Cell viability, apoptotic patterns, reactive oxygen species (ROS) production, Bcl-2/caspase-3 activity, and cell numbers were measured to evaluate the anti-apoptotic and antioxidative effects of memantine.Memantine treatment significantly improved cell viability and reduced cisplatin-induced apoptosis in auditory cells. Bcl-2/caspase-3 activity was also significantly increased, suggesting anti-apoptotic effects against cisplatin-induced ototoxicity.Our results suggest that memantine protects against cisplatin-induced ototoxicity in vitro, providing a potential new strategy for preventing hearing loss in patients undergoing cisplatin chemotherapy.Copyright © 2024, Otology & Neurotology, Inc.