原发性中枢神经系统淋巴瘤伴致密纤维化与不伴致密纤维化免疫微环境下免疫细胞和α平滑肌肌动蛋白阳性细胞的比较分析。
Comparative analyses of immune cells and alpha-smooth muscle actin-positive cells under the immunological microenvironment between with and without dense fibrosis in primary central nervous system lymphoma.
发表日期：2024 Aug 26
作者：
Jun Takei, Miku Maeda, Nei Fukasawa, Masaharu Kawashima, Misayo Miyake, Kyoichi Tomoto, Shohei Nawate, Akihiko Teshigawara, Tomoya Suzuki, Yohei Yamamoto, Hiroyasu Nagashima, Ryosuke Mori, Ryoko Fukushima, Satoshi Matsushima, Hiroyoshi Kino, Ai Muroi, Takao Tsurubuchi, Noriaki Sakamoto, Kaichi Nishiwaki, Shingo Yano, Yuzuru Hasegawa, Yuichi Murayama, Yasuharu Akasaki, Masayuki Shimoda, Eiichi Ishikawa, Toshihide Tanaka
来源：
Brain Structure & Function
摘要：
原发性中枢神经系统淋巴瘤（PCNSL）的组织病理学检查显示，血管周围区域有淋巴细胞同心聚集并伴有纤维化。然而，“僵硬”PCNSL 中这种纤维化的性质仍不清楚。我们遇到过一些 PCNSL 手术发现有硬块。本研究调查了有或没有僵硬的 PCNSL 的致密纤维状态和肿瘤微环境。我们通过银浸渍评估了 9 个具有硬度的 PCNSL 和 26 个无硬度的 PCNSL。 9 例僵硬 PCNSL 中的 6 例表现出明显纤维化的病理特征，其特征是网状纤维聚集和胶原蛋白积聚。比较纤维性 PCNSL 和对照 PCNSL 之间作为癌症相关成纤维细胞的α平滑肌肌动蛋白 (αSMA) 阳性梭形细胞、T 淋巴细胞和巨噬细胞的数量。纤维性 PCNSL 在肿瘤内和肿瘤外环境中均包含丰富的 αSMA 阳性细胞（分别为 5/6、87% 和 3/6、50%）。相反，七个对照 PCNSL 中只有一个在肿瘤内区域含有 αSMA 阳性细胞。此外，肿瘤外αSMA阳性细胞的存在与T淋巴细胞和巨噬细胞的浸润有关。总之，认识到 PCNSL 中致密纤维化的存在可以提供对肿瘤微环境的深入了解。这些结果可能有助于对 PCNSL 患者进行分层并改善这些患者的免疫治疗。© 2024。作者。
Histopathologic examinations of primary central nervous system lymphoma (PCNSL) reveal concentric accumulation of lymphocytes in the perivascular area with fibrosis. However, the nature of this fibrosis in "stiff" PCNSL remains unclear. We have encountered some PCNSLs with hard masses as surgical findings. This study investigated the dense fibrous status and tumor microenvironment of PCNSLs with or without stiffness. We evaluated by silver-impregnation nine PCNSLs with stiffness and 26 PCNSLs without stiffness. Six of the nine stiff PCNSLs showed pathological features of prominent fibrosis characterized by aggregation of reticulin fibers, and collagen accumulations. Alpha-smooth muscle actin (αSMA)-positive spindle cells as a cancer-associated fibroblast, the populations of T lymphocytes, and macrophages were compared between fibrous and control PCNSLs. Fibrous PCNSLs included abundant αSMA-positive cells in both intra- and extra-tumor environments (5/6, 87% and 3/6, 50%, respectively). Conversely, only one out of the seven control PCNSL contained αSMA-positive cells in the intra-tumoral area. Furthermore, the presence of extra-tumoral αSMA-positive cells was associated with infiltration of T lymphocytes and macrophages. In conclusion, recognizing the presence of dense fibrosis in PCNSL can provide insights into the tumor microenvironment. These results may help stratify patients with PCNSL and improve immunotherapies for these patients.© 2024. The Author(s).