结直肠癌 (CRC) 是全球第三大常见癌症，也是癌症相关死亡的第二大主要原因。最近，新兴研究强调了全细胞凋亡（PANoptosis）在肿瘤发展和抗肿瘤治疗中的作用。在本研究过程中，我们仔细鉴定了CRC中与PANoptosis相关的差异表达基因（CPAN_DEGs）与免疫细胞比例之间的相关性分析。随后，我们根据 CPAN_DEG 制定了预后评分。进一步我们的分析显示，经 qRT-PCR 测定验证，HCT116、HT29 和 SW480 细胞内 UNC5D mRNA 表达显着减少。此外，仔细检查 TCGA 数据库揭示了一个独特的趋势，其中 UNC5D 表达低的个体与 UNC5D 水平高的个体相比，总体生存率显着降低。进一步对UNC5D进行药敏分析，结果表明UNC5D与CRC对6-硫鸟嘌呤的敏感性相关。我们的调查结果强调了 PANoptosis 影响免疫失调以及 CRC 预后结果的机制。版权所有：© 2024 张等人。这是一篇根据知识共享署名许可条款分发的开放获取文章，允许在任何媒体上不受限制地使用、分发和复制，前提是注明原始作者和来源。

Colorectal cancer (CRC) ranks as the third most prevalent cancer globally and stands as the second principal contributor to cancer-related fatalities. Recently, emerging research has emphasized the role of pan apoptosis (PANoptosis) in tumor development and anti-tumor therapy. In the course of this investigation, we meticulously identified and conducted a correlation analysis between differentially expressed genes associated with PANoptosis in CRC (CPAN_DEGs) and the proportion of immune cells. Subsequently, we formulated a prognostic score based on the CPAN_DEGs. Further our analysis revealed a noteworthy reduction in UNC5D mRNA expression within HCT116, HT29 and SW480 cells, as validated by qRT-PCR assay. Furthermore, scrutinizing the TCGA database unveiled a distinctive trend wherein individuals with the low UNC5D expression exhibited significantly reduced overall survival compared to their counterparts with the high UNC5D levels. The drug susceptibility analysis of UNC5D was further performed, which showed that UNC5D was corassociated with the sensitivity of CRC to 6-Thioguanine. The outcomes of our investigation underscore the mechanisms by which PANoptosis influences immune dysregulation as well as prognostic outcome in CRC.Copyright: © 2024 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.