金银花是一种一年生真菌，专门寄生在活的忍冬植物上，为开发新资源食品和药物提供了巨大的潜力。然而，该真菌的野生资源和菌丝产量有限，其抗肿瘤活性成分和机制尚不清楚，阻碍了该真菌的开发。为此，我们对金银花发酵培养基进行了优化，并研究了其菌丝体的抗肿瘤活性。结果表明，最佳发酵培养基组成为：2%蔗糖、0.2%蛋白胨、0.1% KH2PO4、0.05% MgSO4·7H2O、0.16%金银花花瓣、0.18% P真菌激发子、0.21%金银花茎。在优化的培养基中培养15天后，生物量达到7.82±0.41 g/l，与马铃薯葡萄糖肉汤培养基相比增加了142%，培养时间缩短了64%。细胞内醇提取物对A549和Eca-109细胞的抑制作用高于细胞内水提取物，半数抑制浓度值分别为2.42和2.92 mg/ml。醇提物分级萃取，得到石油醚相、氯仿相、乙酸乙酯相、正丁醇相。其中，石油醚相比阳性对照表现出更好的效果，半数抑制浓度为113.3 μg/ml。流式细胞术分析表明石油醚成分可诱导Eca-109细胞凋亡，表明该提取成分可作为功能性食品中的抗癌剂。该研究为促进金银花的综合开发和高效利用提供了宝贵的技术支撑和理论基础。

Phylloporia lonicerae is an annual fungus that specifically parasitizes living Lonicera plants, offering significant potential for developing new resource food and medicine. However, wild resources and mycelium production of this fungus is limited, and its anti-tumor active ingredients and mechanisms remain unclear, hampering the development of this fungus. Thus, we optimized the fermentation medium of P. lonicerae and studied the anti-tumor activity of its mycelium. The results indicated that the optimum fermentation medium consisted of 2% sucrose, 0.2% peptone, 0.1% KH2PO4, 0.05% MgSO4·7H2O, 0.16% Lonicera japonica petals, 0.18% P fungal elicitor, and 0.21% L. japonica stem. The biomass reached 7.82 ± 0.41 g/l after 15 days of cultivation in the optimized medium, a 142% increase compared with the potato dextrose broth medium, with a 64% reduction in cultivation time. The intracellular alcohol extract had a higher inhibitory effect on A549 and Eca-109 cells than the intracellular water extract, with half-maximal inhibitory concentration values of 2.42 and 2.92 mg/ml, respectively. Graded extraction of the alcohol extract yielded petroleum ether phase, chloroform phase, ethyl acetate phase, and n-butanol phase. Among them, the petroleum ether phase exhibited a better effect than the positive control, with a half-maximal inhibitory concentration of 113.3 μg/ml. Flow cytometry analysis indicated that petroleum ether components could induce apoptosis of Eca-109 cells, suggesting that this extracted component can be utilized as an anticancer agent in functional foods. This study offers valuable technical support and a theoretical foundation for promoting the comprehensive development and efficient utilization of P. lonicerae.