肿瘤细胞上的程序性死亡配体 1 (PD-L1) 表达与多种恶性肿瘤的不良预后相关，而结直肠癌 (CRC) 的结果则部分矛盾且不确定。本研究旨在通过比较三种不同的抗体克隆来评估 PD-L1 作为 CRC 的预后生物标志物。回顾性纳入了 2007 年 1 月 1 日至 2015 年 12 月 31 日在瑞典卡尔马县接受 CRC 手术的患者。使用克隆 73-10、SP263 和 22C3 评估来自 862 个未经新辅助治疗的原发性肿瘤的组织微阵列，以评估肿瘤细胞 (TC) 和免疫细胞 (IC) 中 PD-L1 的免疫组织化学表达。 Cox回归比例风险模型用于估计单变量和多变量分析中总生存期（OS）和无病间隔（DFI）的风险比，分别将1%和5%设置为TC和IC中阳性表达的截止值TC 中发现 PD-L1 表达，克隆 73-10 为 89 例（10%），克隆 SP263 为 76 例（9%），克隆 22C3 为 38 例（4%），而 IC 中的 PD-L1 表达为 317 例（37克隆 73-10 为 %) 例，克隆 SP263 为 264 (31%) 例，克隆 22C3 为 89 (10%) 例。在所有三个克隆的单变量分析中，IC 中的 PD-L1 表达与延长的 OS 和 DFI 相关。 73-10 和 SP263 与延长 DFI 的联系仍然存在于 73-10 和 SP263 的多变量分析中，但仅适用于 73-10 的 OS。在任何分析中，TC 中的 PD-L1 表达都不能预测 OS，而它与 SP263 的 DFI 延长相关，并且在 73-10 中出现趋势。 SP263 与延长 DFI 的联系仍然存在，而 73-10 在多变量分析中得到了加强。 IC 和 TC 中 PD-L1 表达的预后价值在抗体克隆之间有所不同，73-10 和 SP263 的预后信息比切除的 CRC 中的 22C3。© 2024。作者。

Programmed death-ligand 1 (PD-L1) expression on tumor cells is associated with poor prognosis in several malignancies, while partly contradictory and inconclusive results have been presented for colorectal cancer (CRC). This study aimed to evaluate PD-L1 as a prognostic biomarker in CRC by comparing three different antibody clones.Patients surgically treated for CRC between January 1st, 2007, and December 31st, 2015, in Kalmar County, Sweden, were retrospectively included. Tissue microarrays from 862 primary tumors without neoadjuvant treatment were assessed for immunohistochemical expression of PD-L1 in tumor cells (TC) and immune cells (IC) using clones 73-10, SP263, and 22C3. Cox regression proportional hazard models were used to estimate hazard ratios for overall survival (OS) and disease-free interval (DFI) in univariable and multivariable analyses, with 1% and 5% set as cut-offs for positive expression in TC and IC respectively.PD-L1 expression in TC was found in 89 (10%) cases for clone 73-10, 76 (9%) for clone SP263, and 38 (4%) for clone 22C3, while the numbers for IC were 317 (37%) cases for clone 73-10, 264 (31%) for clone SP263, and 89 (10%) for clone 22C3. PD-L1 expression in IC was associated with prolonged OS and DFI in univariable analysis for all three clones. The link to prolonged DFI remained in multivariable analysis for 73-10 and SP263, but only for 73-10 regarding OS. PD-L1 expression in TC was not prognostic of OS in any analysis, while it was associated with prolonged DFI for SP263, and a trend was seen for 73-10. The link to prolonged DFI remained for SP263 and was strengthened for 73-10 in multivariable analysis.The prognostic value of PD-L1 expression in both IC and TC differs between antibody clones, with 73-10 and SP263 being more reliable for prognostic information than 22C3 in resected CRC.© 2024. The Author(s).