类器官的干细胞。
Stem cells for organoids.
发表日期：2022 Dec
作者：
Shutong Qian, Jiayi Mao, Zhimo Liu, Binfan Zhao, Qiuyu Zhao, Bolun Lu, Liucheng Zhang, Xiyuan Mao, Liying Cheng, Wenguo Cui, Yuguang Zhang, Xiaoming Sun
来源：
Stem Cell Research & Therapy
摘要：
类器官是模拟器官结构和功能的三维 (3D) 细胞培养系统，涉及疾病建模、药物筛选和细胞发育生物学中的应用。类器官中的材料基质可以为干细胞分化成不同细胞类型并不断自我更新提供3D环境，从而实现器官的体外培养，近年来受到广泛关注。然而，类器官仍然存在成熟度低、异质性高、缺乏时空调控等挑战。因此，本文总结了干细胞类器官的培养方案和各种应用，并针对目前干细胞类器官工程化的不足，提出了干细胞类器官工程化的深刻思考，以实现干细胞和工程化类器官的进一步应用和临床转化。类器官研究中的干细胞。© 2022 作者。 Wiley‐VCH GmbH 代表中国科学院大学温州研究院出版的《智慧医疗》。
Organoids are three-dimensional (3D) cell culture systems that simulate the structures and functions of organs, involving applications in disease modeling, drug screening, and cellular developmental biology. The material matrix in organoids can provide a 3D environment for stem cells to differentiate into different cell types and continuously self-renew, thereby realizing the in vitro culture of organs, which has received extensive attention in recent years. However, some challenges still exist in organoids, including low maturity, high heterogeneity, and lack of spatiotemporal regulation. Therefore, in this review, we summarized the culturing protocols and various applications of stem cell-derived organoids and proposed insightful thoughts for engineering stem cells into organoids in view of the current shortcomings, to achieve the further application and clinical translation of stem cells and engineered stem cells in organoid research.© 2022 The Authors. Smart Medicine published by Wiley‐VCH GmbH on behalf of Wenzhou Institute, University of Chinese Academy of Sciences.