简介：乳腺癌是最常见的恶性肿瘤之一，其及时诊断大大增加了成功治疗的前景。大约七分之一的女性一生中都会经历乳腺癌诊断。基质细胞及其分泌因子对肿瘤生长发挥多种作用，影响增殖、侵袭和转移。研究强调了脂肪组织分泌的蛋白质对乳腺癌增殖的显着影响，超过了其他细胞类型释放的因子的影响。然而，肿瘤微环境中参与脂肪因子表达的特定转录因子和辅助因子仍然是个谜。方法：在本研究中，培养脂肪细胞并将其暴露于 980 nm 和 650 nm 光生物调节。 MDA-MD-231 细胞（三阴性癌细胞系）用激光处理细胞的条件培养基培养。采用实时测定来分析与细胞凋亡相关的基因表达水平的变化。结果：结果显示，980 nm 和 650 nm 照射的条件培养基导致癌细胞的细胞活力降低。相反，来自受辐射细胞的条件培养基引发了 Caspase 3、Caspase 9 和 BAX2 基因表达的增加，同时 BCL2 基因表达的减少。结论：研究结果强调了激光处理的条件培养基诱导癌细胞凋亡途径的潜力，为进一步研究利用低强度激光疗法治疗乳腺癌提供了有希望的途径。版权所有 © 2024 J Lasers Med Sci。

Introduction: Breast cancer ranks among the most prevalent malignancies, and its prompt diagnosis significantly amplifies the prospects of successful treatment. Approximately one in seven women will experience a breast cancer diagnosis in their lifetime. Stromal cells and their secreted factors exert various effects on tumor growth, impacting proliferation, invasion, and metastasis. Research has emphasized the significant impact of proteins secreted by adipose tissue on breast cancer proliferation, surpassing the influence of factors released by other cell types. Yet, the specific transcription factors and cofactors involved in adipokine expression in the tumor microenvironment remain enigmatic. Methods: In this study, adipocyte cells were cultured and exposed to 980 nm and 650 nm Photobiomodulation. The MDA-MD-231 cells (triple negative cancer cell line) were cultured with a conditioned medium from laser-treated cells. The real-time assay was employed to analyze the gene expression level changes involved in apoptosis. Results: Results showed that the irradiated conditioned medium at 980 nm and 650 nm caused a reduction in cell viability of cancer cells. Conversely, the conditioned medium from the irradiated cells triggered an increase in the expression of Caspase 3, Caspase 9, and BAX2 genes, alongside a decrease in BCL2 gene expression. Conclusion: The findings highlighted the potential of the laser-treated conditioned medium to induce apoptosis pathways in cancer cells, demonstrating a promising avenue for further research in utilizing low-level laser therapy in breast cancer treatment.Copyright © 2024 J Lasers Med Sci.