限制效应增强双极电化学：结构颜色编码与无线电化学发光成像技术相结合。
Confinement Effect Enhanced Bipolar Electrochemistry: Structural Color Coding Coupled with Wireless Electrochemiluminescence Imaging Technology.
发表日期：2024 Aug 27
作者：
Xiao-Yan Wang, Sheng-Tong Wu, Yi-Zhi Lin, Shou-Nian Ding, Jing-Juan Xu, Hong-Yuan Chen
来源：
BIOSENSORS & BIOELECTRONICS
摘要：
在这项工作中，通过将CNT掺杂到SiO2光子晶体中构建了SiO2/CNTs光子晶体珠，该光子晶体具有与角度无关的响应结构颜色，并且由于其良好的导电性可以用作双极电极。此外，双极电极电化学发光（BPE-ECL）实验和有限元模拟证明，低驱动电压可以通过约束效应触发双极电极电化学反应。受此启发，首次将SiO2/CNTs结构颜色编码方案与基于微通道限域效应的低驱动电压诱导无线BPE-ECL成像相结合，实现卵巢癌生物标志物（CA125、CEA、法新社）。成功构建的高通量BPE-ECL生物传感器对AFP、CEA、CA125的检测限分别为0.72 ng/mL、0.95 ng/mL、1.03 U/mL，具有良好的稳定性和特异性，拓展了应用范围为电化学发光编码技术的发展奠定了基础。
In this work, SiO2/CNTs photonic crystal beads were constructed by doping CNTs into SiO2 photonic crystals, which have an angle-independent responsive structural color and can be used as bipolar electrodes due to their good electrical conductivity. In addition, the bipolar electrode-electrochemiluminescence (BPE-ECL) experiments and finite element simulation prove that the low driving voltage can trigger the bipolar electrode electrochemical reactions by confinement effect. Inspired by this, it is the first to combine the SiO2/CNTs structural color coding scheme with low-drive voltage induced wireless BPE-ECL imaging based on the confinement effect of microchannels to achieve simultaneous immune detection of ovarian cancer biomarkers (CA125, CEA, AFP). The detection limits of successfully constructed high-throughput BPE-ECL biosensor for AFP, CEA, and CA125 are 0.72 ng/mL, 0.95 ng/mL, and 1.03 U/mL, respectively, and have good stability and specificity, which expands the application of electrochemiluminescence and lays a foundation for the development of electrochemiluminescence coding technology.