钙调磷酸酶1（RCAN1）的调节因子在心脏、肝脏、大脑和肾脏等多个器官中表达，与心血管疾病、唐氏综合症和阿尔茨海默病的发病机制密切相关。它还与各种器官肿瘤的发展有关；然而，其在肝细胞癌（HCC）中的潜在作用仍知之甚少。因此，本研究的目的是通过生物信息学分析探讨RCAN1在HCC中的潜在机制。我们基于NCBI和TCGA数据库进行联合分析，结合批量转录组和单细胞分析来检查RCAN1的主要生物学功能HCC 中的 RCAN1 及其与表型、代谢和细胞通讯相关的作用。随后，建立了RCAN1过表达细胞系，并通过体外实验验证了RCAN1对肿瘤细胞的作用。此外，我们致力于通过分子对接和分子动力学模拟来鉴定潜在的相关药物。发现RCAN1在19种癌组织中表达下调，在11种癌组织中表达上调。 RCAN1 表达水平越高，患者生存率越高。 RCAN1主要表达于肝细胞、巨噬细胞、内皮细胞和单核细胞中，其高表达不仅与HCC表型相关细胞的分布密切相关，而且与HCC细胞本身的分布密切相关。此外，Rcan1可能直接或间接参与丙氨酸、天冬氨酸、谷氨酸代谢以及丁酸代谢等代谢途径，从而影响肿瘤细胞的增殖和迁移。体外实验证实RCAN1过表达促进细胞凋亡，同时抑制HCC细胞的增殖和侵袭。通过1615种药物的分子对接，我们筛选出溴苯那明作为潜在的靶点药物，并通过分子动力学验证了我们的结果。本研究通过生物信息学方法揭示了RCAN1与HCC的关系，验证了RCAN1可以通过影响疾病的进展。实验，最终通过药物分子对接和分子动力学鉴定出潜在的治疗药物。© 2024。作者。

The regulator of calcineurin 1 (RCAN1) is expressed in multiple organs, including the heart, liver, brain, and kidney, and is closely linked to the pathogenesis of cardiovascular diseases, Down syndrome, and Alzheimer's disease. It is also implicated in the development of various organ tumors; however, its potential role in hepatocellular carcinoma (HCC) remains poorly understood. Therefore, the objective of this study was to investigate the potential mechanisms of RCAN1 in HCC through bioinformatics analysis.We conducted a joint analysis based on the NCBI and TCGA databases, integrating both bulk transcriptome and single-cell analyses to examine the principal biological functions of RCAN1 in HCC, as well as its roles related to phenotype, metabolism, and cell communication. Subsequently, an RCAN1-overexpressing cell line was established, and the effects of RCAN1 on tumor cells were validated through in vitro experiments. Moreover, we endeavored to identify potential related drugs using molecular docking and molecular dynamics simulations.The expression of RCAN1 was found to be downregulated in 19 types of cancer tissues and upregulated in 11 types of cancer tissues. Higher levels of RCAN1 expression were associated with improved patient survival. RCAN1 was predominantly expressed in hepatocytes, macrophages, endothelial cells, and monocytes, and its high expression not only closely correlated with the distribution of cells related to the HCC phenotype but also with the distribution of HCC cells themselves. Additionally, Rcan1 may directly or indirectly participate in metabolic pathways such as alanine, aspartate, and glutamate metabolism, as well as butanoate metabolism, thereby influencing tumor cell proliferation and migration. In vitro experiments confirmed that RCAN1 overexpression promoted apoptosis while inhibiting proliferation and invasion of HCC cells. Through molecular docking of 1615 drugs, we screened brompheniramine as a potential target drug and verified our results by molecular dynamics.In this study, we revealed the relationship between RCAN1 and HCC through bioinformatics methods, verified that RCAN1 can affect the progress of the disease through experiments, and finally identified potential therapeutic drugs through drug molecular docking and molecular dynamics.© 2024. The Author(s).