目的探讨核输出蛋白1（XPO1）抑制剂selinexor对急性髓系白血病（AML）Kasumi-1细胞增殖抑制和凋亡的影响，采用MTS法检测不同浓度selinexor的抑制效果。不同时间点Kasumi-1细胞增殖的影响。流式细胞术检测不同浓度selinexor处理后细胞凋亡率和细胞周期的变化。Selinexor在不同时间点均以浓度依赖性方式抑制Kasumi-1细胞的生长（r 24 h=0.7592，r 48 h= 0.9456，r 72 h=0.9425）。 Selinexor以时间依赖性方式抑制Kasumi-1细胞生长（2.5 μmol/L组中r = 0.9057，5 μmol/L组中r = 0.9897，10 μmol/L组中r = 0.9994）。 Selinexor能够以剂量依赖的方式诱导Kasumi-1细胞凋亡（r=0.9732），并且随着药物浓度的增加，Kasumi-1细胞的凋亡更加明显。 Selinexor处理Kasumi-1细胞后G0/G1期比例显着增加，S期比例显着降低。随着药物浓度的增加，Kasumi-1细胞周期阻滞在G0/G1期的比例增加，细胞合成减少。Selinexor可通过抑制Kasumi-1细胞增殖、诱导细胞凋亡、阻断肿瘤细胞的凋亡来促进肿瘤细胞死亡。细胞周期。

To investigate the effects of selinexor, a inhibitor of nuclear export protein 1 (XPO1) on the proliferation inhibition and apoptosis of Kasumi-1 cells in acute myeloid leukemia (AML).MTS method was used to detect the inhibitory effect of different concentrations of selinexor on the proliferation of Kasumi-1 cells at different time points. The apoptosis rate and cell cycle changes after treatment with different concentration of selinexor were detected by flow cytometry.Selinexor inhibited the growth of Kasumi-1 cells at different time points in a concentration-dependent manner (r 24 h=0.7592, r 48 h=0.9456, and r 72 h=0.9425). Selinexor inhibited Kasumi-1 cells growth in a time-dependent manner (r =0.9057 in 2.5 μmol/L group, r =0.9897 in 5 μmol/L group and r =0.9994 in 10 μmol/L group). Selinexor could induce apoptosis of Kasumi-1 cells in a dose-dependent manner (r =0.9732), and the apoptosis of Kasumi-1 cells was more obvious with the increase of drug concentration. The proportion of G0/G1 phase was significantly increased and the proportion of S phase was significantly decreased after the treatment of Kasumi-1 cells by selinexor. With the increase of drug concentration, the proportion of Kasumi-1 cells cycle arrest in G0/G1 phase was increased and the cell synthesis was decreased.Selinexor can promote the death of tumor cells by inhibiting Kasumi-1 cells proliferation, inducing apoptosis and blocking cell cycle.