类器官技术近十年来发展迅速，涉及发育、再生及多种疾病机制的探索，且涉及多个学科交叉。细胞生物学、组织工程、纳米科学、肿瘤学等研究领域已经发表了数千篇关于3D培养或类器官的文献，导致研究人员及时总结这些研究具有挑战性。文献计量统计是帮助研究人员有效地澄清上述问题并系统地管理整个景观的有用方法。在我们的研究中，2009年1月至2024年5月期间所有有关类器官的原创文章均收录在Web of Science数据库中，并使用Excel软件、R软件的“bibliometrix”软件包、VOSviewer和CiteSpace收集和分析相关信息。结果，总共纳入了 6222 篇论文，对类器官的现状进行了分类并预测了未来的研究领域。我们的研究结果突显了类器官相关出版物的增长趋势，其中美国和荷兰在这一领域处于领先地位。加州大学系统、哈佛大学、乌得勒支大学和乌得勒支大学医学中心已成为关键贡献者，该领域的主要作者包括 Clevers、H、Beekman、JM 和 Spence JR。我们的结果还揭示了类器官的研究热点和趋势主要集中在临床治疗、药物筛选以及“水凝胶”和“微流控技术”等材料和技术在类器官中的应用。接下来，我们深入解读了类器官研究领域的发展历程，包括技术的出现、从实验室到床边的转化、研究最广泛的类器官类型概况、材料和技术的应用等。新兴的类器官免疫共培养趋势。此外，我们还讨论了类器官技术的陷阱、挑战和前景。总之，本研究为读者提供了直接、便捷地进入类器官研究领域的途径。版权所有 © 2024 Qu、Mu、Bi、Chen、Wang、Ma 和 Lu。

Organoid technology has been developed rapidly in the past decade, which involves the exploration of the mechanism of development, regeneration and various diseases, and intersects among multiple disciplines. Thousands of literature on 3D-culture or organoids have been published in the research areas of cell biology tissue engineering, nanoscience, oncology and so on, resulting in it being challenging for researchers to timely summarize these studies. Bibliometric statistics is a helpful way to help researchers clarify the above issues efficiently and manage the whole landscape systematically. In our study, all original articles on organoids were included in the Web of Science database from January 2009 to May 2024, and related information was collected and analyzed using Excel software, "bibliometrix" packages of the R software, VOSviewer and CiteSpace. As results, a total of 6222 papers were included to classify the status quo of the organoids and predict future research areas. Our findings highlight a growing trend in publications related to organoids, with the United States and Netherlands leading in this field. The University of California System, Harvard University, Utrecht University and Utrecht University Medical Center have emerged as pivotal contributors and the key authors in the field include Clevers, H, Beekman, JM and Spence JR. Our results also revealed that the research hotspots and trends of organoids mainly focused on clinical treatment, drug screening, and the application of materials and technologies such as "hydrogel" and "microfluidic technology" in organoids. Next, we had an in-depth interpretation of the development process of organoid research area, including the emergence of technology, the translation from bench to bedsides, the profiles of the most widely studied types of organoids, the application of materials and technologies, and the emerging organoid-immune co-cultures trends. Furthermore, we also discussed the pitfalls, challenges and prospects of organoid technology. In conclusion, this study provides readers straightforward and convenient access to the organoid research field.Copyright © 2024 Qu, Mu, Bi, Chen, Wang, Ma and Lu.