随着牛蛙供应量的不断增长，已成为重要的水产养殖品种。由于缺乏可操作的行业标准和法规，牛蛙抗病药物的滥用和肝脏脂质代谢异常已成为牛蛙养殖业发展的主要障碍。谷胱甘肽是一种可在细胞内合成的天然三肽，其生理功能主要包括治疗肝脏疾病、抗氧化、解毒、抗肿瘤、增强免疫力、延缓衰老等。本研究探讨谷胱甘肽对牛蛙的治疗作用通过肝脏脂质代谢和血清代谢组学分析发现肝脏脂质代谢异常。对NC组、FI组和GSH组脂代谢异常牛蛙饲喂谷胱甘肽20 d，对存活率、肝脏组织形态、血清抗氧化酶活性、肝脏脂肪酶活性以及血清代谢组学、肝脏代谢组学进行研究分析。研究表明，谷胱甘肽能够修复肝脏，提高脂质代谢异常牛蛙的存活率；血清SOD酶活性显着升高； ACP、AKP活性显着降低； HDL-C和T-CHO活性显着升高；肝脏中LDL-C、TBA、TG活性显着降低； PC、PS、PE等代谢物含量显着上调，且自噬-其他、坏死性凋亡、ErbB信号通路水平上调，鞘脂代谢、D-氨基酸代谢代谢途径水平下调，在一定程度上下调了鞘脂代谢和D-氨基酸代谢途径，在一定程度上缓解了甘油磷脂和氨基酸代谢的紊乱，从而缓解了肝脏脂质代谢的异常。结果表明，谷胱甘肽可以有效治疗牛蛙肝脏脂质代谢紊乱，促进牛蛙生长发育，修复肝功能，减轻炎症，促进牛蛙产业健康绿色发展。版权所有 © 2024 苏高舒蔡方、雷、李、胡。

With the continuous growth of bullfrog supply, it has become an important aquaculture species. Due to the lack of actionable industry standards and regulation, the misuse of anti-disease drugs and abnormal liver lipid metabolism in bullfrogs have become a major obstacle to the development of bullfrog aquaculture industry. Glutathione is a natural tripeptide that can be synthesized intracellularly, and its physiological functions mainly include the treatment of liver diseases, antioxidant, detoxification, anti-tumor, enhancement of immunity, and delaying aging.In this study, the therapeutic effect of glutathione on bullfrogs with abnormal liver lipid metabolism was revealed from hepatic lipid metabolism and serum metabolomics analysis. The survival rate, liver histomorphology, serum antioxidant enzyme activity, liver lipase activity and serum metabolomics, liver metabolomics were studied and analyzed by feeding the bullfrogs with abnormal lipid metabolism with glutathione for 20 days in the NC, FI and GSH groups.The results of the study showed that glutathione was able to repair the liver and improve the survival rate of bullfrogs with abnormal lipid metabolism; the activity of serum SOD enzymes was significantly increased; the activities of ACP and AKP were significantly decreased; the activities of HDL-C and T-CHO were significantly increased; and the activities of LDL-C, TBA, and TG were significantly decreased in the liver; the contents of metabolites, such as PC, PS, and PE were significantly up-regulated, and the levels of up-regulated Autophagy - other, Necroptosis and ErbB signaling pathway, and down-regulated Sphingolipid metabolism, D-Amino acid metabolism metabolic pathway, to some extent The metabolic pathways of Sphingolipid metabolism and D-Amino acid metabolism were down-regulated to alleviate the disorders of glycerophospholipid and amino acid metabolism to a certain extent, thus alleviating the abnormalities of liver lipid metabolism.The results showed that glutathione could effectively treat the liver lipid metabolism disorder of bullfrogs, promote the growth and development of bullfrogs, repair the liver function, reduce the inflammation, and promote the healthy and green development of bullfrog industry.Copyright © 2024 Su, Gao, Shu, Cai, Fang, Lei, Li and Hu.