本研究利用黍最大总提取物（PMTE）的地上部分以环保的方式合成银纳米颗粒（AgNP）。使用 TEM、SEM、FTIR、X 射线粉末衍射 (XRD)、Zeta 电位、UV 和 FTIR 对绿色银纳米颗粒 (PM-AgNPs) 进行表征。与 (PMTE) 相比，PM-AgNPs 对乳腺癌 (MCF-7)、肺 (A549) 和卵巢腺癌 (SKOV3) 人类肿瘤细胞的抗癌作用进行了评估。 AgNPs 对金黄色葡萄球菌分离株的抗菌活性进行了评估。 PM-AgNPs的吸光度为418nm，粒径为15.18nm，zeta电位为-22.4mV，保证了纳米银的稳定性。 XRD 评估了形成的 PM-AgNP 的晶体学性质。 PM-AgNPs 对 MCF-7 和 SKOV 3 的细胞毒性最强，与 A549 (13±3.2μg/mL) 相比，IC50 分别为 0.13±0.015 和 3.5±0.5μg/ml。 MCF-7和SKOV3细胞系的凋亡细胞增加分别为97.79±1.61和96.6±1.91%。 PM-AgNPs 被发现分别影响 50% 和 43.75% 的测试分离株的膜完整性和膜通透性。此外，PM-AgNPs 还减少了金黄色葡萄球菌生物膜的形成。这些结果表明使用 PM-AgNP 来治疗乳腺癌和卵巢癌。

This study uses the aerial parts of Panicum maximum total extract (PMTE) to synthesize silver nanoparticles (AgNPs) in an environmentally friendly manner. TEM, SEM, FTIR, X-ray powder diffraction (XRD), Zeta potential, UV, and FTIR were used to characterize the green silver nanoparticles (PM-AgNPs). PM-AgNPs were evaluated as anticancer agents compared to (PMTE) against breast (MCF-7), lung (A549), and ovary adenocarcinoma (SKOV3) human tumour cells. The antibacterial activity of AgNPs was assessed against Staphylococcus aureus isolates. The PM-AgNPs had an absorbance of 418 nm, particle size of 15.18 nm, and zeta potential of -22.4 mV, ensuring the nanosilver's stability. XRD evaluated the crystallography nature of the formed PM-AgNPs. The cytotoxic properties of PM-AgNPs on MCF-7 and SKOV 3 were the strongest, with IC50s of 0.13 ± 0.015 and 3.5 ± 0.5 g/ml, respectively, as compared to A549 (13 ± 3.2 µg/mL). The increase in the apoptotic cells was 97.79 ± 1.61 and 96.6 ± 1.91% for MCF-7 and SKOV3 cell lines, respectively. PM-AgNPs were found to affect the membrane integrity and membrane permeability of 50 and 43.75% of the tested isolates, respectively. Also, PM-AgNPs have recorded a reduction in the biofilm formation of S. aurues. These results suggest using PM-AgNPs to treat breast and ovarian cancers.