用于表面增强拉曼散射（SERS）成像的金基纳米粒子在精确肿瘤检测和光热治疗（PTT）方面显示出巨大潜力。然而，金纳米颗粒（Au NP）的代谢能力引起了人们的极大关注。在此，我们设计了一种表面聚乙二醇化的硫化铜（CuS）包覆的金纳米粒子的核壳纳米结构（PEG-Au@CuS NSs）。注射 PEG-Au@CuS NSs 的小鼠 1 h 和 4 h 胆囊中 Au 的排泄量分别是相同 Au 粒径的聚乙二醇化 Au NPs (PEG-AuNPs) 的 8.2 倍和 19.1 倍。通过在 PEG-Au@CuS NSs 的核壳连接处加载拉曼报道分子 3,3'-二乙基硫代三碳花青碘化物 (DTTC)，PEG-Au-DTTC@CuS NSs 表现出拉曼信噪比 (S/N) ）在携带原位 CT26-Luc 结肠肿瘤的小鼠中静脉注射 (IV) 24 小时后，比率为 4.01。相比之下，DTTC 涂层的 PEG-AuNP（PEG-Au-DTTC NP）的信噪比为 2.71。此外，与 808 nm 激光激发的 PEG-Au-DTTC NP 相比，PEG-Au-DTTC@CuS NSs 表现出增强的光热转换效应。 PEG-Au-DTTC@CuS NSs 使术中 SERS 图像引导光热疗法能够完全治愈结肠肿瘤小鼠。我们的数据表明，PEG-Au-DTTC@CuS NSs 是有前途的术中拉曼图像引导治疗诊断纳米平台，具有增强的肝胆排泄作用。

Gold-based nanoparticles for surface-enhanced Raman scattering (SERS) imaging show great potential for precise tumor detection and photothermal therapy (PTT). However, the metabolizability of gold nanoparticles (Au NPs) raises big concerns. Herein, we designed a core-shelled nanostructure of copper sulfide (CuS)-coated Au NPs with surface pegylation (PEG-Au@CuS NSs). The excreted Au in the gallbladders at 1 h and 4 h in mice injected with PEG-Au@CuS NSs was 8.2- and 19.1-fold of that with the pegylated Au NPs (PEG-AuNPs) of the same Au particle size, respectively. By loading the Raman reporter 3,3'-Diethylthiatricarbocyanine iodide (DTTC) in the core-shell junction of PEG-Au@CuS NSs, the PEG-Au-DTTC@CuS NSs exhibited the Raman signal-to-noise (S/N) ratio of 4.01 after 24 h of intravenous (IV) injection in the mice bearing an orthotopic CT26-Luc colon tumor. By contrast, the DTTC-coated PEG-AuNPs (PEG-Au-DTTC NPs) achieved an S/N ratio of 2.71. Moreover, PEG-Au-DTTC@CuS NSs exhibited an increased photothermal conversion effect compared with PEG-Au-DTTC NPs excited with an 808-nm laser. PEG-Au-DTTC@CuS NSs enabled intraoperative SERS image-guided photothermal therapy for a complete cure of the colon tumor-bearing mice. Our data demonstrated that the PEG-Au-DTTC@CuS NSs are promising intraoperative Raman image-guided theranostic nanoplatform with enhanced hepatobiliary excretion.