背景：空间和功能性肝脏分区是由沿肝脏门脉中心轴的异质组织建立的，对于确保最佳肝功能非常重要。研究人员试图开发可靠的肝脏模型来模拟肝脏微环境，并使用在开发的系统中培养的肝细胞分析肝功能。然而，由于模型系统中缺乏可灌注的血管网络以及随着时间的推移维持肝细胞功能的限制，在体外模拟肝脏微环境仍然是一个巨大的挑战。方法：在本研究中，我们建立了一个在连续冲洗介质流下运行的微生理系统，从而向肝脏类器官供应营养和氧气，并从中清除废物并释放细胞因子，类似于血管的功能。结果：微生理系统应用于类器官培养有利于缩短人肝脏类器官的分化时间，提高其功能成熟度。结论：因此，我们的微生理培养系统可能会开启将小型化肝脏模型系统集成到单个设备中的可能性，从而实现更合理的肝脏反应体外测定。版权所有 © 2024 Byeon、Jung、Han、Son 和 Jeong。

Background: Spatial and functional hepatic zonation, established by the heterogeneous tissue along the portal-central axis of the liver, is important for ensuring optimal liver function. Researchers have attempted to develop reliable hepatic models to mimic the liver microenvironment and analyze liver function using hepatocytes cultured in the developed systems. However, mimicking the liver microenvironment in vitro remains a great challenge owing to the lack of perfusable vascular networks in the model systems and the limitation in maintaining hepatocyte function over time. Methods: In this study, we established a microphysiological system that operated under continuous flush medium flow, thereby allowing the supply of nutrients and oxygen to liver organoids and the removal of waste and release of cytokines therefrom, similar to the function of blood vessels. Results: The application of microphysiological system to organoid culture was advantageous for reducing the differentiation time and enhancing the functional maturity of human liver organoid. Conclusion: Hence, our microphysiological culture system might open the possibility of the miniaturized liver model system into a single device to enable more rational in vitro assays of liver response.Copyright © 2024 Byeon, Jung, Han, Son and Jeong.