卵巢癌（OC）是人类生殖系统中主要的原发肿瘤。唾液酸化异常对肿瘤的发展、转移、免疫逃避、血管生成和治疗抵抗具有显着影响。 B4GALT5是一种与唾液酸化相关的基因，在卵巢癌中发挥着至关重要的作用，可能会影响临床病理特征和预后。我们对TIMER、GEPIA2、GeneMANIA和Metascape进行了全面检索，从The Cancer获得了卵巢癌的转录谱数据基因组图谱（TCGA）。免疫组化检测B4GALT5的表达。为了研究 B4GALT5 对 OC 细胞生长和程序性细胞死亡的影响，我们进行了 Transwell 测定和蛋白质印迹。B4GALT5 的存在与 OC 中的不利结果密切相关。 B4GALT5显着促进OC细胞增殖。通过分析基因本体论 (GO) 和京都基因与基因组百科全书 (KEGG)，发现 B4GALT5 在细胞外基质中发挥着至关重要的作用，特别是在含有胶原蛋白的结构中，并表现出与 ECM 受体相互作用、转录失调的相关性癌症以及白细胞介素 1 受体信号通路。此外，B4GALT5 与 OC 中的肿瘤免疫微环境之间存在明显的联系。此外，B4GALT5 在多种类型的癌症中表现出良好的表达水平，包括 CHOL、KIRC、STAD 和 UCES。 总之，人们普遍认为 B4GALT5 在 OC 的生长和进展中发挥着关键作用，其高表达是不利结果的指标。此外，B4GALT5 积极参与塑造 OC 内的癌症免疫微环境。这项研究有可能大大有助于更深入地了解 B4GALT5 在人类恶性肿瘤中的实质性参与，特别是 OC。© 2024。作者。

Ovarian cancer (OC) is the predominant primary tumor in the human reproductive system. Abnormal sialylation has a significant impact on tumor development, metastasis, immune evasion, angiogenesis, and treatment resistance. B4GALT5, a gene associated with sialylation, plays a crucial role in ovarian cancer, and may potentially affect clinicopathological characteristics and prognosis.We conducted a comprehensive search across TIMER, GEPIA2, GeneMANIA, and Metascape to obtain transcription profiling data of ovarian cancer from The Cancer Genome Atlas (TCGA). The expression of B4GALT5 was test by immunohistochemistry. To investigate the impact of B4GALT5 on growth and programmed cell death in OC cells, we performed transwell assays and western blots.The presence of B4GALT5 was strongly associated with an unfavorable outcome in OC. B4GALT5 significantly promoted the proliferation of OC cells. Upon analyzing gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), it was discovered that B4GALT5 played a crucial role in the extracellular matrix, particularly in collagen-containing structures, and exhibited correlations with ECM-receptor interactions, transcriptional dysregulation in cancer, as well as the interleukin-1 receptor signaling pathway. Furthermore, there is a clear link between B4GALT5 and the tumor immune microenvironment in OC. Moreover, B4GALT5 exhibits favorable expression levels across various types of cancers, including CHOL, KIRC, STAD and UCES.In conclusion, it is widely believed that B4GALT5 plays a pivotal role in the growth and progression of OC, with its heightened expression serving as an indicator of unfavorable outcomes. Moreover, B4GALT5 actively participates in shaping the cancer immune microenvironment within OC. This investigation has the potential to contribute significantly to a deeper understanding of the substantial involvement of B4GALT5 in human malignancies, particularly OCs.© 2024. The Author(s).