活性氧 (ROS) 的过量或突然产生会诱发氧化应激，引发一系列危重疾病，包括癌症、帕金森病和缺血再灌注损伤。传统的 ROS 生物测定涉及捕获、标记和光谱检测等离散步骤，既复杂又耗时。此外，ROS 的寿命短（微秒到毫秒），严重影响了它们的准确性。因此，迫切需要一种能够实现实时检测的快速有效的方法。在这项研究中，我们通过将糊剂直接沉积到柔性聚对苯二甲酸乙二醇酯 (PET) 基材上，开发了一种基于坚固的纳米酶复合材料的可印刷柔性 ROS 传感器。该设备展示了对实验室中的过氧化氢（模拟剂）和个人汗液中总 ROS 的快速实时响应，在 0.01-10 mM 的宽浓度范围内表现出对过氧化氢的出色电流响应，检测限 (LOD) 为 1.85 μM。该设备对过氧化氢的灵敏度 (136.59 μA mM-1 cm-2) 比之前报道的传感器高 1.5 至 10 倍。此外，IFRS设备在体外成功识别了成年男性汗液中的瞬时ROS水平，半小时剧烈运动后电流响应增加了8倍，表现出优异的选择性、显着的稳定性，并证实了高生物安全性。总体而言，IFRS 为不久的将来简单、快速和实时的 ROS 检测提供了可行且实用的解决方案。版权所有 © 2024 作者。由 Elsevier B.V. 出版。保留所有权利。

Excessive or burst generation of reactive oxygen species (ROS) can induce oxidative stress, precipitating a range of critical illnesses, including cancers, Parkinson's disease and Ischemia-reperfusion injury. Conventional biological assays for ROS, involving discrete steps of capturing, labelling, and spectrometric detection, are complex and time-intensive. Moreover, their accuracy is substantially compromised by the short lifespan (microseconds to milliseconds) of ROS. Consequently, there is a pressing need for a rapid and efficient method that enables real-time detection. In this study, we have developed a printable, flexible ROS sensor based on a robust nanoenzyme composite by direct deposition of the paste onto a flexible polyethylene terephthalate (PET) substrate. This device demonstrated the fast and real-time responses to the hydrogen peroxide (mimetic agent) in the laboratory and to total ROS in sweat of an individual, exhibiting an outstanding current response to hydrogen peroxide across a broad concentration range of 0.01-10 mM, with a limit of detection (LOD) of 1.85 μM. The device's sensitivity to hydrogen peroxide (136.59 μA mM-1 cm-2), was found to be 1.5 to 10 times higher than that of sensors previously reported. Moreover, the IFRS device successfully identified instantaneous ROS levels in the sweat of adult males in vitro, with amperometric response increased 8 times after half an hour strenuous exercise, thereby exhibiting excellent selectivity, remarkable stability, and confirmed high biosafety. Overall, the IFRS provides a viable and practical solution for simple, expedited, and real-time ROS detection in the near future.Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.